Use of sunflower immature embryos culture in in vitro for fast creation of fertility restorer resistant to tribenuron methyl herbicide

  • V. O. Babych
  • O. I. Varchenko
  • M. V. Kuchuk
  • M. F. Parii
  • Ya. F. Parii
  • Yu. V. Symonenko
DOI: https://doi.org/10.7124/FEEO.v27.1297

Abstract

Aim. Acceleration of the sunflower lines homozygosity for isolation of the fertility restorer resistant to tribenuron methyl herbicide using in vitro culture of sunflower immature embryos. Methods. Methods of immature sunflower embryos cultivation in in vitro culture; methods of classical crossing. Results. As a result, homozygous genotype was created and isolated from three combinations (BH0118xSURES-2, BH0218xSURES-2 and BH0318xSURES-2) for 10 parent sunflower lines. Conclusions. The use of the technology of sunflower immature embryos cultivation in in vitro culture effectively accelerates the sunflower lines homozygosity for the fertility restorer isolation resistant to tribenuron methyl herbicide. The involvement of immature sunflower embryos cultivation in in vitro culture methods can reduce time for creation of initial parental sunflower lines twice.

Keywords: sunflower, immature embryos, in vitro culture, herbicide resistance, tribenuron methyl.

References

Seiler G.J., Qi L.L., Marek L.F. Utilization of sunflower crop wild relatives for cultivated sunflower improvement. Crop Sci. 2017. Vol. 57. P. 1083–1101. doi: 10.2135/cropsci2016.10.0856.

Vear F. Changes in sunflower breeding over the last fifty years. Oilseeds & fats crops and lipids (OCL). 2016. Vol. 23, No. 2. D202, P. 1–8. doi: 10.1051/ocl/2016006.

Leclercq P. Une sterilité mâle cytoplasmique chez le tournesol. Ann. Amelior. Plant. 1969. Vol. 19. P. 99–106.

Demurin J.N., Pihtjareva A.A., Tronin A.S. Peredacha gene ustoichivosti k tribenuron-metilu v selekcionniy material podsolnechnika VNIIMK. Nauchno-technicheskiy bulleten Vserosiyskogo nauchno-issledovatelskogo instituta maslichnih kultur. 2013. Vol. 1. P. 153–154. [in Russian]

Olson B.L.S., Al-Khatib K., Aiken R.M. Distribution of resistance to imazamox and tribenuron-methyl in native sunflower. National sunflower association. 2004. Accessed from: http://www.sunflowernsa.com/research/resea rch-workshop/documents/158.pdf

Miller J.F., Al-Khatib K. Registration of two oilseed sunflower genetic stocks, SURES -1 and SURES -2, resistant to tribenuron herbicide. Crop Science. 2004. Vol. 44. P. 1037–1038.

Notice of release of two sulfonylurea herbicide resistant sunflower genetic stocks. Accessed from: https://www.ag.ndsu.edu/fss/ndsu-varieties/fact-sheets-and-brochures/SURES12.pdf

Kolkman J.M., Slabaugh M.B., Bruniard J.M., Berry S., Bushman B.S., Olungu C., Maes N., Abratti G., Zambelli A., Miller J.F., Leon A., Knapp S.J. Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower. Theor. Appl. Genet. 2004. Vol. 109. P. 1147–1159. doi: 10.1007/s00122-004-1716-7.

Sala C.A., Bulos M., Echarte A.M., Whitt S.R., Ascenzi R. Molecular and biochemical characterization of an induced mutation conferring imidazolinone resistance in sunflower. Theor. Appl. Genet. 2008. Vol. 118, No. 1. P 105–112. doi: 10.1007/s00122-008-0880-6.

Sala C.A., Bulos M., Alteri E., Ramos M.L. Genetics and breeding of herbicide tolerance in sunflower. Helia. 2012. Vol. 35, No. 57. P. 57–70. doi: 10.2298/HEL1257057S.

Finer J. Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium. Plant Cell Reports. 1987. Vol. 6, No. 5. P. 372–374. doi: 10.1007/BF00269564.

Malone-Schoneberg J., Scelonge C.J., Burrus M., Bidney D.L. Stable transformation of sunflower using Agrobacterium and split embryonic axis explants. Plant Science. 1994. Vol. 103, No. 2. P 199–207. doi: 10.1016/0168-9452(94)90208-9.

Montathong K., Machikowa T., Muangsan N. Cytological and food reserve changes in sunflower cotyledons in vitro. Suranaree Journ. of Scien. & Technol. 2019. Vol. 26, No. 2. P. 141-150.

Lucas O., Kallerhoff J., Alibert G. Production of stable transgenic sunflowers (Helianthus annuus L.) from wounded immature embryos by particle bombardment and co-cultivation with Agrobacterium tumefaciens. Molecular Breeding. 2000. Vol. 6. P. 479–487. doi: 10.1023/A:1026583931327.

Soroka A., Lyakh V. Genetic variability in sunflower after mutagen treatment of immature embryos of different ages. Helia. 2009. Vol. 32, No. 51. P. 33-45. doi: 10.2298/HEL0951033S.

Hewezi T., Rerrault A., Alibert G., Kallerhoff J. Dehydrating immature embryo split apices and rehydrating with Agrobacterium tumefaciens: A new method for genetically transforming recalcitrant sunflower. Plant Molecular Biology Reporter. 2002. Vol. 20. P. 335–345. doi: 10.1007/BF02772121.