Protein status of winter wheat cell lines with combined tolerace to abiotic stresses
Aim. Winter wheat cell lines were selected on medium with the addition of lethal doses of Cd2+ cations. Those variants demonstrated stable growth under osmotic stress pressure. The aim of the investigation was the estimation of cell protein status during cultivation under various conditions. Methods. Cd-resistant cell lines of winter wheat (cv. Favoritka) were cultivated under normal conditions and on cultural media with addition of lethal doses of manitol or Cd2+ (stresses I, II). From calli tissues crude protein matter was obtained and detected by Laemmli system electrophoresis. Results. Cd-resistant winter cell lines showed stable growth under any abiotic stress pressure. Electrophoregrams demonstrated peculiar features of some protein fraction accumulation that correlated with the age of cultures and type of stress agent. Conclusions. There were no decrease of total protein pools in calli tissues during cell cultivation under any (manitol, Cd2+) lethal stress pressure. The quantitative accumulation of some protein fractions were correlated with the type of stress agent. This event was especially manifested in variants, cultivated on media with manitol addition. Protein status of Cd-resistant cell lines of winter wheat is the reflection of their combined stress tolerance.
Keywords: winter wheat, cell selection, cadmium ions, osmotic stress, tolerant cell line, protein.
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