Protein status of winter wheat cell lines with combined tolerace to abiotic stresses

  • L. E. Sergeeva
  • M. O. Dykun
  • L. I. Bronnikova
DOI: https://doi.org/10.7124/FEEO.v26.1276

Abstract

Aim. Winter wheat cell lines were selected on medium with the addition of lethal doses of Cd2+ cations. Those variants demonstrated stable growth under osmotic stress pressure. The aim of the investigation was the estimation of cell protein status during cultivation under various conditions. Methods. Cd-resistant cell lines of winter wheat (cv. Favoritka) were cultivated under normal conditions and on cultural media with addition of lethal doses of manitol or Cd2+ (stresses I, II). From calli tissues crude protein matter was obtained and detected by Laemmli system electrophoresis. Results. Cd-resistant winter cell lines showed stable growth under any abiotic stress pressure. Electrophoregrams demonstrated peculiar features of some protein fraction accumulation that correlated with the age of cultures and type of stress agent. Conclusions. There were no decrease of total protein pools in calli tissues during cell cultivation under any (manitol, Cd2+) lethal stress pressure. The quantitative accumulation of some protein fractions were correlated with the type of stress agent. This event was especially manifested in variants, cultivated on media with manitol addition. Protein status of Cd-resistant cell lines of winter wheat is the reflection of their combined stress tolerance.

Keywords: winter wheat, cell selection, cadmium ions, osmotic stress, tolerant cell line, protein. 

References

Sidorov V.A. Biotekhnologia rastenii. Kiev: Naukova dumka, 1990. 280 p. [in Russian]

Sergeeva L.E. Kletochnaya selektsia s ionami tyazholyh metallov dlya polucheniya genotipov rastenii s kompleksnoi ustoichivostiu k abioticheskim streesam. Kiev: Logos, 2013. 211 p. [in Russian]

Qiang G., Zhai X.-G., Han Z.-X. Cloning and sequence analysis of new gene coding drought tolerance, LEA3 from Tibet hullless barley. Zuowu xuebao=Acta Agr. Sin. 2007. Vol. 33. P. 292–296.

Tioleter D., Jaquinod M., Mangavel C., Passirani C., Saulner P., Manon S., Teyssier E., Payet N., Avelange-Macherel M.-H., Macherel D. Structure and function of a mitochondrial late embryogenesis abundant protein by desiccation. Plant Cell. 2007. Vol. 19. P. 1580–1587. doi: 10.1105/tpc.107.050104

Hu X-y., Tan X.-f., Tian X.-m. Cloning kDNA, sequences and presumed physiological role of dehydrin-liked protein from Camellia oleifera. Xibei zhiwu xuebao= Acta Bot. Boreali-occid. Sin. 2008. Vol. 28 (8). Р. 1541–1548.

Seriogin I.V., Ivanov V.B. Fiziologicheskie aspekty toksicheskogo deistviya kadmiya i svintsa na vysshie rasteniya. Fiziologiya rastenii. 2002. Vol. 48. P. 606–630. [in Russian]

Gamborg J.L., Miller R.A., Ojima K. Nutrient requirement of suspension cultures of soybean roots. Exp. Cell Res. 1968. Vol. 509. P. 151–158. doi: 10.1016/0014-4827(68)90403-5

Laemmli V.K. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature. 1970. Vol. 227. P. 52–59. P.680. doi: 10.1038/227680a0

Sergeeva L., Dykun M., Bronnikova L. Biotekhnologia pshenytsi. Proteinovyi pul tkanyn roslyn i klitynnykh kultur. Naukovyi visnyk Skhidnoevropeiskogo natsionalnogo universitetu im. Lesi Ukainky Biologichni nauky. 2017. No. 13 (362). P. 32–36. [in Ukrainian] doi: 10.29038/2617-4723-2017-362-13-32-36

Terletskaya N.V. Nespetsificheskie reaktsii zernovykh zlakov na abioticheskie stressy in vivo i in vitro. Almaty, 2012. 207 p. [in Russian]

Nesterenko O.G., Litvinov S.V., Rashydov N.M. The protein expression changes during the signaling systems interaction in stressed pea seedlings. Faktory eksperymentalnoi evoliutsii organizmiv. 2018. Vol. 22. P. 154–161. [in Ukrainian] doi: 10.7124/FEEO.v22.941