Polyploidization of Miscanthus sinensis using dinitroanilines with low phytotoxicity

  • O. V. Melnychuk
  • S. P. Ozheredov
  • D. B. Rakhmetov
  • S. O. Rakhmetova
  • O. O. Bayer
  • O. M. Shysha
  • A. I. Yemets
  • Ya. B. Blume


Aim. The aim of the work was to obtain polyploid lines of Miscanthus sinensis with enhanced biomass productivity using new dinitroaniline compounds. Methods. The work was carried out in in vitro conditions. Aseptic M. sinensis shoots were cultured on shoot multiplication medium containing various compounds of dinitroanilines class. Classic dinitroanilines (oryzalin and trifluralin) were used as a control. Results. When aseptic shoots were cultured on medium supplemented with oryzalin and trifluralin at concentration 10 мM, survival rate of explants was between 23.52 and 44.40%. However, when the medium was supplemented with tested dinitroanilines at the same concentration, survival rate of explants was in the range of 95.0–96.5%. Conclusions. Possibility of new compounds of dinitroanilines class utilization for M. sinensis polyploidization has been studied. In terms of explants survival rate, application of new compounds of dinitroanilines class appeared to be significantly effective than application of classic dinitroanilines.

Keywords: dinitroanilines, in vitro, polyploidization, Miscanthus sinensis.


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