Polyploidization of Miscanthus sinensis using dinitroanilines with low phytotoxicity
Abstract
Aim. The aim of the work was to obtain polyploid lines of Miscanthus sinensis with enhanced biomass productivity using new dinitroaniline compounds. Methods. The work was carried out in in vitro conditions. Aseptic M. sinensis shoots were cultured on shoot multiplication medium containing various compounds of dinitroanilines class. Classic dinitroanilines (oryzalin and trifluralin) were used as a control. Results. When aseptic shoots were cultured on medium supplemented with oryzalin and trifluralin at concentration 10 мM, survival rate of explants was between 23.52 and 44.40%. However, when the medium was supplemented with tested dinitroanilines at the same concentration, survival rate of explants was in the range of 95.0–96.5%. Conclusions. Possibility of new compounds of dinitroanilines class utilization for M. sinensis polyploidization has been studied. In terms of explants survival rate, application of new compounds of dinitroanilines class appeared to be significantly effective than application of classic dinitroanilines.
Keywords: dinitroanilines, in vitro, polyploidization, Miscanthus sinensis.
References
Heaton E.A., Dohleman F.G., Miguez A.F., Juvik J.A., Lozovaya V., Widholm J., Zabotina O.A., McIsaac F., David M.B., Voight T.B., Boersma N.N., Long S.P. Miscanthus: a promising biomass crop. Adv. Bot. Res. 2010. Vol. 56. P. 75-135. doi: 10.1016/B978-0-12-381518-7.00003-0
Melnychuk O.V., Ozheredov S.P., Rakhmetov D.B., Rakhmetova S.O., Sekan A.S., Bayer G.Y., Shysha O.M., Yemets A. I. In vitro culture establishment and polyploidization of Miscanthus giganteus. Sci. Reports of NULES of Ukraine. 2015. Vol. 57(8). [in Ukrainian] Accessed from: http://nd.nubip.edu.ua/2015_8/8.pdf
Yemets A., Blume Ya. Progress in plant polyploidization based on antimicrotubular drugs. The Open Horticulture J. 2008. Vol. 1 (1). P. 15-20. doi: 10.2174/1874840600801010015
Zhang K., Wang, X, Cheng, F., 2019. Plant polyploidy: origin, evolution, and its influence on crop domestication. Hortic. Plant J. 2019. Vol. 5 (6). P. 231-239. doi: 10.1016/j.hpj.2019.11.003
Shumnyy V.K. Novaia forma miskantusa kitayskogo (veernika kitayskogo Miscanthus sinensis Anders.) kak perspektivnyy istochnik tselliulozosoderzhashchego syr'ia. Vestnik VOGiS. 2010. Vol. 14 (1). P. 122-126. [in Russian]
Melnychuk O.V., Оzheredov S.P., Sekan O.S., Bayer G.Ya., Shysha O.M., Yemets А.І. Development and application of method for miscanthus in vitro culture establishment. Faktori eksperimental'noi evolucii organizmiv. 2015. Vol. 17. P. 209- 212. [in Ukrainian]
Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 1962. No. 15. P. 473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x
Gubisova M., Gubis J., Zofajova A., Mihalik D., Kraic J. Enhanced in vitro propagation of Miscanthus x giganteus. J Ind Crop Prod. 2013. No. 41. P. 279-282. doi: 10.1016/j.indcrop.2012.05.004
Melnychuk O.V., Оzheredov S.P., Rakhmetov D.B., Yemets А.І., Blume Ya.B. Screening of nitroanilines for their affinity to miscanthus α-tubulin for further in vitro polyploidization of miscanthus species. Faktori eksperimental'noi evolucii organizmiv. 2016. Vol. 18. P. 212-216. [in Ukrainian]
Kundelchuk O.P., Tarasenko L.V., Blume Ya.B. Comparison of effect of amiprofosmetil on the structure of root cells in sensitive and resistant lines of Nicotiana plumbaginifolia (in Russ.), Russ. J. Plant Pysiol. 2002. Vol. 49 (3). P. 425-430. doi: 10.1023/A:1015501304476
Pausheva Z.P. Workshop on Plant Cytology (in Russ.). Moscow: Agropromizdat, 1988.
Yu C.Y., Kim H.S., Burn A.L., Widholm J.M., Juvik J.A. Chromosome doubling of the bioenergy crop, Miscanthus giganteus. Global Change Biol. Bioenergy. 2009. Vol. 1. P. 404-412. doi: 10.1111/j.1757-1707.2010.01032.x
Petersen K., Hagberg P., Kristiansen K. In vitro chromosome doubling of Miscanthus sinensis. Plant Breed. 2002. Vol. 121. P. 445-450. doi: 10.1046/j.1439-0523.2002.738314.x
Petersen K., Hagberg P., Kristiansen K. Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis. Plant Cell Tiss. Org. Cult. 2003. Vol. 73. P. 137-146.
