Grain barley starch as a gelling agent of nutrient media for cultivation of anthers and isolated embryos of H. vulgare L.

  • O. V. Bilynska

Abstract

Aim. Determination the possibility of agar replacement with normal and waxy type starches produced from barley seeds in the nutrient media for spring barley haploid production. Methods. Anthers of spring barley line DH00-126 were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose (9.0 %) and differed in solidifying agents (agar, starch from barley seeds or chemically modified starch D5a-1). Trophic capacities of barley starch were determined both in anther and embryo culture of barley. Results. It was determined that barley starch of waxy type was not suitable for solidifying of nutrient medium. Use of normal type barley starch (6.5 %) resulted in sufficient decrease in the frequency of green plant regeneration. At the same time, this modification had no effect on the number of morphogenic anthers. Barley starch was inferior to pea starch in the ability to support seedling growth in embryo culture in vitro. Conclusions. Barley starch of normal type has worse trophic capacities in comparison to pea starch in embryo culture. It was appeared barley starch to be a less suitable gelling agent of medium for spring barley haploid production in anther culture in vitro than agar and chemically modified starch D5a-1.

Keywords: Hordeum vulgare L., anther culture in vitro, embryo culture in vitro, agar, barley starch.

References

Sorvari S. Differentiation of potato tuber disks in barley starch gelatinized nutrient media. Ann. Agric. Fenn. 1986b. Vol. 25. P. 133‒138.

Bilynska O.V. Effect of nutrient media containing natural and chemically modified starches on haploid production in spring barley anther culture in vitro. Biological Systems, Biodiversity, and Stability of Plant Communities / Ed. L.I. Weisfeld, A.I. Opalko, N.A. Bome, S.A. Beguzarova. Waretown (USA): Apple Academic Press, 2015. P. 211−228.

Sorvari S. The effect of starch gelatinized nutrient media in barley anther culture. Ann. Agr. Fenn. 1986а. Vol. 25. P. 127–133.

Finnie S.J., Powell W., Dyer A.F. The effect of carbohydrate composition and concentration on anther culture response in barley (Hordeum vulgare L.) Plant Breed. 1989. Vol. 103 (2). P. 110‒118. doi: 10.1111/j.1439-0523.1989.tb00358.x.

Kuhlmann U., Foroughi-Wehr B. Production of doubled haploids in frequencies sufficient for barley breeding programs. Plant Cell Rept. 1989. Vol., No. 2. P. 110–118. doi: 10.1007/BF00716843.

Sorvari S., Schider O. Influence of sucrose and melibiose on barley anther culture in starch media. Plant Breed. 1987. Vol. 99. No. 2. P. 161‒171. doi: 10.1111/j.14390523.1987.tb01167.x.

Sorvari S. Comparison of anther cultures of barley cultivars in barley starch and agar gelitinized media. Ann. Agr. Fenn. 1986c. Vol. 25. P. 249–254.

Belinskaia E.V., Dul'nev P.H. Modifitsirovannyy krakhmal DKKmod kak komponent pitatel'noy sredy dlia polucheniia gaploidov iachmenia v kul'ture pyl'nikovin vitro. Fiziologiia i biokhimiia kul'turnikh rasteniy. 2007. Vol. 39 (2). P. 136–143. [in Russian]

Bilyns'ka O. V. Zastosuvannia kukurudzianykh krokhmaliv z pidvyshchenym vmistom amilozy (mutatsii ae i su2) uskladi shtuchnoho zhyvyl'noho seredovyshcha dlia oderzhannia haploidiv iaroho iachmeniu u kul'turi pyliakiv in vitro. Visnyk Kharkivs'koho natsional'noho universytetu im. V.N. Karazina. Seriia: biolohiia. 2010. Vol. 11 (905). P. 60–65. [in Ukrainian]

Bilynska O.V., Tymchuk S.M., Derebizova O.Yu. Artificial nutrient medium for barley haploid production in anther culture in vitro: Patent for invention 103426 Ukraine. No. a201210359; applied on 03.09.2012., published 10.10.2013, bulletin No. 19.

Bilynska O.V. Method for production of barley immature embryo culture in vitro: Patent for invention 118385 Ukraine. No. a2017100177; applied on 04.01.2017, published 10.01.2019, bulletin No. 14.

Rikhter M., Augustat Z., Shirbaum K. Izbrannye metody issledovaniia krakhmala. Moscow: Pishchevaia promyshlennost', 1975. 183 p. [in Russian]

Prozina M.N. Botanicheskaia mikrotekhnika. Moscow: Vysshaia shkola, 1960. 205 p. [in Russian]

Bilyns'ka O.V., Dul'niev P.H. Morfohenetychnyy efekt i trofichni vlastyvosti khimichno modyfikovanoho krokhmaliu D-5aM u kul'turi in vitro pyliakiv ta kul'turi izol'ovanykh zarodkiv iachmeniu iaroho. Faktory eksperymental'noi evoliutsii orhanizmiv. 2015. Vol. 17. P. 107–111. [in Ukrainian]