γ-tubulin gene intron length polymorphism of Arabidopsis thaliana

  • A. M. Rabokon
  • Yu. O. Bilonozhko
  • A. S. Postovoitovа
  • L. O. Kalafat
  • Ya. V. Pirko
  • Ya. B. Blume
DOI: https://doi.org/10.7124/FEEO.v26.1253

Abstract

Aims. Verification of the possibility of using the γ-tubulin gene intron length polymorphism method in genetic studies of plants on the example of Arabidopsis thaliana. Methods. The γ-tubulin gene intron length polymorphism evaluating method was used. Amplified fragments DNA were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. Arabidopsis was first time analyzed using the γ-tubulin gene intron length polymorphism method. During amplification with degenerate primers 2 amplicons (520 bp and 555 bp) were formed in all samples. However, using selected arabidopsis-specific primers for the second intron of the γ-tubulin genes, it was possible to find several samples that differ in their DNA profile. Conclusions. It is established that the proposed method can be used in molecular genetic studies of plants. Moreover, the developed specific primers for γ-tubulin gene introns can probably be used both for the study of Arabidopsis and related species. The use of degenerate primers can be useful in the study of plants for which there is no information about their genome.

Keywords: molecular-genetic markers, intron length polymorphism, γ-tubulin, A. thaliana.

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