Genetic background for moderate resistance against fusarium head blight among winter wheat developed in the Forrest Steppe of Ukraine
Aim. The aim of the work was to evaluate the genetic background of resistance to Fusarium head blight in common winter wheat cultivars based on the allelic state of the TDF_076_2D gene conferring tolerance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi. Methods. We studied 91 winter common wheat cultivars developed in the Institute of Plant Physiology and Genetics of NAS of Ukraine. A silica-based commercial kit was used for DNA extraction. For the allelic state detection, the INDEL1 marker co-segregating with the TDF_076_2D gene was used. Results. The frequency of the resistance allele according to the marker for the gene conferring moderate resistance to the Fusarium fungi made up 0.802. Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the gene of interest. The data obtained are consistent with the results of previous research for the wider sample of the winter and spring common wheat cultivars. The cultivars with confirmed resistance allele might show lower infection level in the field and serve as a source of the gene in marker assisted selection.
Keywords: common wheat, disease resistance genes, Fusarium head blight, molecular markers.
Retman S.V., Shevchuk O.V., Gorbacheva N.P. Diseases of leaves and ear. Karantyn i zakhyst roslyn. 2011. No 4. P. 25–27. [in Ukrainian]
Kovalyshina H.M., Murashko L.A., Kovalyshyn A.B. Spike diseases in winter wheat of the Forest Steppe of Ukraine. Visnyk Ukrainskogo tovarystva genetykiv i selektsioneriv. 2008. Vol. 6 (2). P. 223–239. [in Ukrainian]
Grytsiuk N.V. Resistance of winter wheat varieties against Fusarium infections at different infection periods. Karantyn i zahust roslyn. 2013. No 10. P. 1–3. [in Ukrainian]
Yarynchyn A.M. Resistance of winter wheat varieties against Fusarium infections of the spike. Zakhyst i karantyn roslyn. 2009. No 4. P. 13–15. [in Ukrainian]
Babayants O.V. Immunological characteristics of plant genetic resources and substantiation of genetic protection against causative agents of fungal diseases in the Steppe of Ukraine. Author’s abstract of dissertation for the degree of Doctor of biological sciences: 06.01.11. Kyiv, 2011. 50 p. [in Ukrainian]
Leonard K.J., Bushnell W.R. Fusarium head blight of wheat and barley. St. Paul: American Phytopathological Society Press, 2003. 512 p.
Salgado J.D., Madden L.V., Paul P.A. Quantifying the effects of Fusarium head blight on grain yield and test weight in soft red winter wheat. Phytopathology. 2015. Vol. 105 (3). P. 295–306. doi: 10.1094/PHYTO-08-14-0215-R.
Mesterhazy A., Bartok T., Mirocha C.G., Komoroczy R. Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breed. 2008. Vol. 118. P. 97–110.
Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 2005. Vol. 43. P. 205–227. doi: 10.1007/s00122-002-1137-4.
del Blanco I.A., Frohberg R.C., Stack R.W., Berzonsky W.A., Kianian S.F. Detection of QTL linked to Fusarium head blight resistance in Sumai 3-derived North Dakota bread wheat lines. Theor. Appl. Genet. 2003. Vol. 106 (6). P. 1027–1031. doi: 10.1007/s00122-002-1137-4.
Cuthbert P.A., Somers D.J., Thomas J., Cloutier S., Bruln-Babel A. Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 2006. Vol. 112 (8). P. 1465–1472. doi: 10.1007/s00122-006-0249-7.
Golkari S., Gilbert J., Ban T., Procunier J.D. QTL-specific microarray gene expression analysis of wheat resistance to Fusarium head blight in Sumai-3 and two susceptible NILs. Genome. 2009. Vol. 52 (5). P. 409–418. doi: 10.1139/g09-018.
Zhu Q.H., Stephen S., Kazan K., Jin G., Fan L., Taylor J., Dennis E.S., Helliwell C.A., Wang M.B. Characterization of the defense transcriptome responsive to Fusarium oxysporum-infection in Arabidopsis using RNA-seq. Gene. 2013. Vol. 512. P. 259–266. doi: 10.1016/j.gene.2012.10.036.
Gao C.S., Kou X.J., Li H.P., Zhang J.B., Saad A.S.I., Liao Y.‐C. Inverse effects of Arabidopsis NPR1 gene on Fusarium seedling blight and Fusarium head blight in transgenic wheat. Plant Pathol. 2013. Vol. 62 (2). P. 383–392. doi: 10.1111/j.1365-3059.2012.02656.x.
Diethelm M., Schmolke M., Groth J., Friedt W., Schweizer G., Hartl L. Association of allelic variation in two NPR1-like genes with Fusarium head blight resistance in wheat. Mol Breeding. 2014. Vol. 34 (1). P. 31–43. doi: 10.1007/s11032-013-0010-2.
Kozub N.A., Sozinov I.A., Karelov A.V., Blume Ya.B., Sozinov A.A. Diversity of Ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes. Cytol Genet. 2017. Vol. 51 (2). P. 117–129. doi: 10.3103/S0095452717020050.
Karelov A., Kozub N., Sozinov I., Sozinova O., Mavromatis A.G., Xynias I.N. Molecular detection of resistance to biotic stress conditions in spring bread wheat cultivars. Innovative Approaches and Applications for Sustainable Rural Development, A. Theodoridis et al. (eds.). Springer Earth System Sciences, Springer Nature Switzerland AG, 2019. P. 305–324. doi: 10.1007/978-3-030-02312-6_18.