Genetic profiling of mistletoe (Viscum album L.) using RAPD-analysis

  • Yu. O. Bilonozhko
  • A. N. Rabokon
  • A. S. Postovoitovа
  • L. O. Kalafat
  • S. M. Privаlikhin
  • N.M. Pirko
  • A. Ye. Demkovych
  • Ya. B. Blume
  • Ya. V. Pirko


Aim. The aim of this research was genetic profiling and identification of genetic differences between V. album speciments, growing on deciduous and coniferous species of woody plants using RAPD markers. Methods. The method of polymerase chain reaction (PCR) with random primers (Random Amplification of Polymorphic DNA - RAPD) was used. Amplified DNA fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using the staining with silver nitrate. Results. All the studied mistletoe samples were differentiated from each other, and their unique molecular genetic profiles were obtained. 241 amplified DNA fragments were detected in the range from 200 to 2000 bp, 152 fragments (63%) were polymorphic. The samples were divided into two separate groups depending on the type of host plant. Conclusions. The fact that the samples formed two separate clades confirms the assumption that mistletoe, which grow on pine and grow on maple, represents two separate subspecies of V. album.

Keywords: Viscum album L., molecular genetic markers, polymorphism, RAPD.


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