Evaluation of genetic diversity of honey bee in Ukraine analyzed by the SSR-markers

  • D. I. Hryhorchuk
  • A. M. Rabokon
  • A. S. Postovoitovа
  • N. M. Pirko
  • Ya. V. Pirko
  • Ya. B. Blume


Aim. The aim of the work was to analyze current genetic structure of honey bee populations in Ukraine that belong to different subspecies: A. meliffera meliffera, A. meliffera carnica, A. meliffera macedonica using microsatellite markers. Methods. SSR-analysis was used for evaluation of the honey bee polymorphism. Amplified fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The analysis of the sample of honey bees (workers and male-bees) collected from different regions of Ukraine was performed by using two SSR-markers (Ac011 and A007). In this sample reasonably high polymorphism was observed, especially for the SSR-marker A007. Conclusions. It was estimated that SSR-analysis method can be applied in molecular-genetic analysis of honey bees for evaluation of genetic diversity and cross-subspecies hybridization.

Keywords: microsatellite markers, Apis meliffera, PIC (Рolymorphism Іnformation Сontent).


Ruttner, F. Biogeography and Taxonomy of Honeybees. Berlin, Heidelberg: Springer Berlin Heidelberg. 1988.

Alpatov V. Porody medonosnoy pchely. 1-e izd. Moskva, 1948. [in Russian]

Dall'Olio R., Marino A., Lodesani M., Moritz R. Genetic characterization of Italian honeybees, Apis mellifera ligustica, based on microsatellite DNA polymorphisms. Apidologie. 2007. Vol. 38 (2). P. 207–217. doi:10.1051/apido:2006073.

Štastny M., Gasper J., Bauer M. Genetic structure of Apis mellifera carnica in Slovakia based on microsatellite DNA polymorphism. Biologia. 2017. Vol. 72 (11). P. 1341–1346. doi: 10.2298/ABS141102048N.

Polishchuk V., Metlitskaja E., Losev O., Golovetsky I. Genetical criteria purebred and features of population frame of the Ukrainian breed bees. Naukovi dopovidi NUBiP. 2012. No 8 (30). [in Ukrainian] Accessed from: http://www.nbuv.gov.ua/ejournals/Nd/2012_1/12moi.pdf.

Cherevatov O.V., Panchuk I.I., Kerek S.S. et al. Molecular diversity of the CoI–CoII spacer region in the mitochondrial genome and the origin of the carpathian bee. Cytol. Genet. 2019. Vol. 53. P. 276–281. doi: 10.3103/S0095452719040030.

Bilash G., Krivtsov N. Selektsiia pchel. Moskva: Agropromizdat. 1991. 304 p. [in Russian]

Brovars'kyy V., Brindza Ya., Otchenashko V., Povoznikov M., Adamchuk, L. Metodyka doslidnoi spravy u bdzhil'nytstvi. 1st publ. Kyiv: Publishing house “Vinichenko”, 2017. P. 155–158. [in Ukrainian]

Williams J., Hanafey M., Rafalski J., Tingey, S. Genetic analysis using random amplified polymorphic DNA markers. Recombinant DNA Methodology II. 1995. P. 849–884. doi: 10.1016/0076-6879(93)18053-f.

Zietkiewicz E., Rafalski A., Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 1994. Vol. 20(2). P.176-183. doi: 10.1006/geno.1994.1151.

Evans J., Schwarz R., Chen Y., Budge G. et al. Standard methods for molecular research in Apis mellifera. J. Apicult. Res. 2013. Vol. 52 (4). P. 1–54. doi: 10.3896/IBRA.

Katti M., Ranjekar P., Gupta V. Differential distribution of simple sequence repeats in eukaryotic genome sequences. Mol. Biol. Evolution. 2001. Vol. 18 (7). P. 1161–1167. doi: 10.1093/oxfordjournals.molbev.a003903.

Solignac M., Vautrin D., Loiseau A., Mougel F. et al. Five hundred and fifty microsatellite markers for the study of the honeybee (Apis mellifera L.) genome. Mol. Ecol. Notes. 2003. Vol. 3 (2). P. 307–311. doi: 10.1046/j.1471-8286.2003.00436.x.

Solignac M., Mougel F., Vautrin, D. Monnerot M., Cornuet J. Third-generation microsatellite-based linkage map of the honey bee, Apis mellifera, and its comparison with the sequence-based physical map. Genome Biol. 2007. Vol. A8 (4). P. R66. doi: 10.1186/gb-2007-8-4-r66.

Doyle J., Doyle J. CTAB DNA extraction in plants. Phytochem. Bull. 1987. Vol. 19 (1). P. 11–15.

Shaibi T., Lattorff H., Moritz R. A microsatellite DNA toolkit for studying population structure in Apis mellifera. Mol. Ecol. Resour. 2008. Vol. 8 (5). P. 1034–1036. doi: 10.1111/j.1755-0998.2008.02146.x.

Green M., Sambrook J., Sambrook J. Molecular cloning. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2012.

Benbouza H., Jean-Marie J., Jean-Pierre B. Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol. Agron. Soc. Environ. 2006. Vol. 10 (2). P. 77–81.

Kondratyuk A.V., Kilchevsky A.V., Kuzminova E.I. Microsatellite loci polymorphism analysis of Belarusіian and foreign breeding potato varieties. Mol. Appl. Genetics (Minsk). 2005. Vol. 13. P. 24–29.