Manifestation of hybrid dysgenesis in Drosophila melanogaster Harwich line with tetracycline suppressed internal microflora
Aim. To evaluate the dynamics of the manifestation of hybrid dysgenesis in the Harwich line observed under the influence of tetracycline and compare the gathered data with the data for the Oregon-R line. Methods. The line of Drosophila melanogaster Harwich was investigated. This line is considered the standard line of the P cytotype. Removal of Wolbachia from the body was implemented using antibiotic tetracycline. The level of hybrid dysgenesis was studied by the method of extracting gonad flies and assessing their condition. Results. A stable and significant increase in the proportion of dysgenic individuals within the Harwich line was recorded after suppression of the internal microflora - including Wolbachia pipientis – by tetracycline. Conclusions. The suppression of the internal microflora of Drosophila melanogaster by tetracycline significantly affects the level of manifestation of hybrid dysgenesis caused by transpositions of the P-element. The data obtained are similar to the results of a similar study of the level of manifestation of hybrid dys-
genesis caused by transpositions of hobo MGE.
Keywords: mobile genetic elements, hybrid dysgenesis, P-element, Drosophila melanogaster, Wolbachia pipientis.
Vasil'eva L.A., Antonenko O.V., Zakharov I.K. The role of mobile genetic elements in the genome of Drosophila melanogaster. Vavilovskiy zhurnal genetiki i selektsii. 2011. Vol. 15(2). P. 225–260. [in Russian]
Pimpinelli S., Berloco M., Fanti L., Dimitri P., Bonaccorsi S., Marchetti E., Caizzi R., Caggese C., Gatti M. Transposable elements are stable structural components of Drosophila melanogaster heterochromatin. Proceedings of the National Academy of Sciences of the USA. 1995. Vol. 92. P. 3804–3808. doi: 10.1073/pnas.92.9.3804
Fry A.J., Palmer M.R., Rand D.M. Variable fitness effects of Wolbachia infection in Drosophila melanogaster. Heredity. 2004. P. 1–11. doi: 10.1038/sj.hdy.6800514
Weeks A.R., Turelli M., Harcombe W.R., Reynoilds K.T., Hoffmann A.A. From parasite to mutualist: rapid evolution of Wolbachia in natural popylations of Drosophila. PLoS Biology. 2007. Vol. 5(7). P. 997–1005. doi: 10.1371/journal.pbio.0050114
Yurchenko N.N., Kovalenko L.V., Zakharov I.K. Transposable elements: instability of genes and genomes. Vavilovskiy zhurnal genetiki i selektsii. 2011. Vol. 15(2). P. 261–270. [in Russian]
Kozeretska I.A. Drosophila melanogaster transposable elements: distribution in natural populations. Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv. 2010. Vol. 8(1). P. 81–90. [in Russian]
Kravets А.Р., Mousseau T.A., Omel’chenko Zh.A., Vengjen G.S. Dynamics of hybrid dysgenesis frequency in Drosophila melanogaster in controlled terms of protracted radiation exposure. Cytology and genetics. 2010. No 1. P. 21–26. [in Russian]
Gorodnyanski I.D., Vorobyova L.I. Effect of suppression of internal microflora by tetracycline on the level of manifestation of the hybrid dysgenesis of Drosophila melanogaster. Factors in experimental evolution of organisms. 2018. Vol. 22. P. 34–39. [in Russian] doi: 10.7124/FEEO.v22.920
Kidwell M.G., Kidwell J.F, Sved J.A. Hybrid dysgenesis in Drosophila melanogaster: a syndrome of aberrant traits including mutation, sterility and male recombination. Genetics. 1977. Vol. 86. P. 813–833.