Some aspects of in vitro wheat biotechnology

  • L. E. Sergeeva
  • L. I. Bronnikova
DOI: https://doi.org/10.7124/FEEO.v25.1184

Abstract

Aim. Drastic climate changes lead to decrease of the appropriate agricultural plants and stimulate the elaboration of new biotechnologies. The preferences of in vitro system are used for providing the acceleration of the plant selection. The cultivating in vitro is a procedure combined common approaches and special adaptation to plant species. This ideology is essential for all cereals and for wheat in particular. There are several aspects of this ideology: the optimization of cultural conditions; the obtaining wheat cultures and studying distinctive features of their proliferation; the detection parameters of viability, realized on the entire plant level; the comparison of those reactions with cells characteristics. Methods. The standard manipulations of primary explants dissection and several protocols of callus induction and raise are used. Results. Cell cultures of new wheat genotypes were obtained. Those forms were selected in the Institute of Plant Physiology and Genetics NAS of Ukraine. The peculiar features of wheat cell cultures were revealed and investigated. Conclusions. Cell cultures obtained from new genotypes of winter wheat demonstrated common reactions with young plants. Parallel investigations of some biochemical parameters realized on cellular level in cell cultures and plant cells is a possible way to acceleration the genotypes with better characteristics selection.

Keywords: winter wheat, in vitro system, cell culture.

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