Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material

  • A. G. Salivon
  • K. V. Lystvan
  • S. V. Litvinov
  • S. A. Pchelovska
  • Yu. V. Shylina
  • V. V. Zhuk
  • L. V. Tonkal
DOI: https://doi.org/10.7124/FEEO.v25.1183

Abstract

Aim. The purpose of this work was to determine the dose of g- and X-ray pre-sowing irradiation of seeds of Hypericum perforatum L., causing an increase in biomass and the accumulation of flavonoids in medicinal raw materials. Methods. X-ray and g-irradiation of seeds, biometric methods, flavonoids extraction, quantification of flavonoid content in extracts using spectrophotometry, qualitative and semi-quantitative analysis of extracts using high-performance liquid chromatography (HPLC), common statistical methods for processing and analysis of data. Results. It was shown that acute X-ray pre-sowing irradiation of H. perforatum seeds at a dose of 20 Gy leads to an increase in the crop of medicinal raw material without losing its pharmaceutical value. Irradiation also increases concentration of quercetin, routine, hyperoside in herbal medicines, grown from seeds, that was chronically irradiated with g-radiation in a total dose of 1 Gy. The HPLC analysis of extracts confirmed that the qualitative composition of ethanol extracts of H. perforatum did not change due to the effect of irradiation on the seeds. Conclusions. The obtained results confirm the possibility of application of pre-sowing irradiation of seeds of H. perforatum in the range of 1–35 Gy in order to increase its productivity and increase the pharmaceutical value of the medicinal raw material.

Keywords: Hypericum perforatum L., ionizing irradiation, productivity, flavonoids.

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