The regeneration potential of promising winter common wheat lines in shoot apical meristem culture

  • S. V. Pykalo
  • O. A. Demydov
  • T. V. Yurchenko
  • N. I. Prokopik
  • O. V. Humeniuk
DOI: https://doi.org/10.7124/FEEO.v25.1181

Abstract

Aim. To investigate the regenerative ability of promising winter common wheat lines in shoot apical meristem culture. Methods. Plant tissue culture methods, statistical evaluation of data. Results. The processes of morphogenesis in culture of apical meristem of 3-days seedlings of lines of winter common wheat were investigated and it was established that the frequency of callusogenesis and shoot regeneration in the studied forms is determined by the genotype of explant. Two types of callus with morphophysiological properties were identified: morphogenic and nonmorphogenic callus. The formation of regenerated plants from wheat calli took place through both gemmorizogenesis and somatic embryogenesis. Conclusions. The line Erytrospermum 60068 was characterized the highest regeneration potential and it can be recommended for further biotechnology of wheat. Obtained technology of vigorous regenerated plant production of winter common wheat lines in shoot apical meristem culture can be used in cell selection and genetic engineering experiments.

Keywords: winter common wheat, apical meristem, genotype, callus, shoot regeneration.

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