Evaluation of some physiological and biochemical parameters of Camelina sativa seedlings under osmotic stress

  • L. V. Nishchenko
  • L. O. Sakhno
DOI: https://doi.org/10.7124/FEEO.v25.1179

Abstract

Aim. To find the most drought resistant spring camelina (Camelina sativa (L.) Crantz) genotype for further involvement in genetic transformation experiments, the some physiological and biochemical parameters of seedlings have been investigated under normal conditions and osmotic stress in vitro. Methods. Sterile seeds were planted on Murashige and Skoog agarized medium without hormones or the same media supplemented with mannitol as an osmotic stress inductor. Germination, fresh weight, total soluble protein content, and superoxide dismutase (SOD) activity have been investigated in 7-day-old seedlings. Results. The analyzed camelina genotypes were differed in their reactions on water deficit in vitro. It has been revealed that higher SOD activity of FEORZhYaF-1 seedlings was accompanied by higher germination and higher ability to fresh weight accumulation under osmotic stress as compared to other varieties. Conclusions. FEORZhYaF-1 breeding form could be involved in genetic transformation experiments to improve drought resistance. SOD activity should be used as a parameter for selection of osmotic stress resistant plant material.

Keywords: Camelina sativa (L.) Crantz  drought resistance, superoxide dismutase, total soluble protein.

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