The creating of intergenetic hybrids of festulolium of Festuca arundinacea morphotipe with the use of post-genomic technologies and DNA-marking

  • I. P. Kondratskaya
  • A. N. Yukhimuk
  • V. A. Stolepchenko
  • O. V. Chizhik
  • Z. G. Kozlovskaya
  • P. P. Vasko
  • V. N. Reshetnikov
DOI: https://doi.org/10.7124/FEEO.v25.1172

Abstract

Aim. To form the varietal population of festulolium intergeneric hybrids of Festuca arundinacea morphotype. To carry out DNA-labeling of created festulolium hybrid plants and parental forms. Methods. The festulolium intergeneric hybrid’s creation was carried out by embryo culture method from an immature caryopsis by growing on a nutrient medium. For the plant genotypes labeling the multilocus primers associated with coding DNA regions (Start Codon Targeted (SCoT) Polymorphism), SRAP (Sequence-related amplified polymorphism) have been selected. Results. The viable plants of intergeneric festulolium hybrids of Festuca arundinacea morphotype have been obtained. To select the best festulolium biotypes for variety populations with high feed and seed productivity formation. A system for hybrid plants genotypes and their parental forms registration in the form of molecular genetic passports have been elaborated. The genetic passports reflects the allele’s composition in loci associated with DNA coding sequences. Conclusions. The best biotypes with economically valuable traits were selected and included in the further selection process. The molecular genetic passports of hybrid festulolium plants and parental forms were composed.

Keywords: festulolium, immature caryopsis, biotypes, DNA, PCR, molecular genetic passports.

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