Efficiency of spring barley haploid production in anther culture in vitro: comparison of basic and improved technologies

  • O. V. Bilinska
  • P. G. Dulnyev
DOI: https://doi.org/10.7124/FEEO.v25.1161

Abstract

Aim. Evaluation of innovative methodological approaches elaborated on model genotypes for usability to increase frequencies of morphogenic structure induction and plant regeneration in anther culture in vitro in spring barley diverse material. Methods. Spikes isolated from F1 and F2 hybrids of four crosses were pretreated using an improved method (4ºC, 28 days), and anthers were inoculated onto nutrient media containing chemically modified starches D5-M and D5a-1 instead of agar. In control cut tillers were emerged in water and pretreated at 4ºC for 5 days. Anthers were cultivated on agar solidified medium. Results. Positive effects of the improved method of cold pretreatment and cultivation of anthers on media solidified with starches were confirmed. The advantage of new gelling agent D5a-1 was proved. Particularly, its usage resulted in a three-fold increase in the frequency of green plant regeneration. Conclusions. In order to increase spring barley androgenic haploid yield, combination of prolonged cold pretreatment with anther cultivation on media solidified with chemically modified starches instead of agar in an integrated technological process is reasonable.

Keywords: Hordeum vulgare L., anther culture in vitro, cold pretreatment, starch, agar, embryo formation, plant regeneration.

References

Foroughi-Wehr B., Mix G. In vitro response of Hordeum vulgare anther culture from plants grown under different environ-ments. Inviron. and Exp. Bot. 1979. Vol. 19. P. 303–309. doi: 10.1016/0098-8472(79)90033-9.

Prakash J, Giles K. Induction and growth of androgenic haploids. International review of cytology. 1987. Vol. 107. P. 273−292.

Asif M. Application and use of haploids. In: Asif M, editor. Progress and opportunities of doubled haploid production. (Swit-zerland): Springer International Publishing, 2013. P. 55−70.

Thomas W.T.B., Forster B.P., Gertsson B. Doubled haploids in breeding. In: Doubled haploid production in crop plants / Ed. Maluszynski M., Kasha K.J., Forster B.P., Szarejko I. Dordrecht: Kluwer academic publishers, 2003. P. 337–349.

Kasha K.J., Simion E., Oro R, Yao Q.A., Hu T.C., Carlson A.R. An improved in vitro technique for microspore culture of barley. Euphytica. 2001. Vol. 120. P. 319–385. doi: 10.1023/A:101756410.

Devaux P., Kasha K. J. Overview of barley doubled haploid production. In: Advances in haploid production in high plants / Ed. Touraev A., Forster B.P., Mohan S.J. Springer Science+Business Media, 2009. P. 47–64.

Germana M.A. Somatic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rept. 2011.Vol. 30. P. 839−857. doi: 10.1007/s00299-011-1061-7.

Dunwell J.M. Haploids in flowering plants: origins and exploitation. Plant Biotechnology Journal. 2010. Vol. 8, No. 4. P. 377–424. doi: 10.1111/j.1467-7652.2009.00498.x.

El Goumi Y., Fakiri M., Benbachir M. et al. Effect of Cold Pretreatment, Anthers Orientation, Spikelet Position and Donor Tiller on the Callusing Response in Barley Anther In vitro Culture. International Journal of Medical Biotechnology and Genetics. 2017. S2 (003). P. 3−38. doi: 10.19070 /2379-1020-SI02003.

Maraschin S.F., De Periester W., Spaink H.P., Wang M. Androgenic switch: an example of plant embryogenesis from the male genotype perspective. Journal of Experimental botany. 2005. Vol. 56. P. 1711−1726. doi: 10.1093/jxb/eri190.

Belinskaya E.V., Dulnyev P.G. Modified starch DKKmod as a component of nutrient medium for barley haploid production in anther culture in vitro. Fiziol. Biokhim. Kult. Rast. 2007. Vol. 39, No. 2. P. 136–143. [in Russian]

Bilynska O.V. Application of corn starches with high amylose content (mutations ae і su2) in nutrient medium for spring bar-ley haploid production in anther culture in vitro. Vіsnik Kharkіvs'kogo nacіonal'nogo unіversitetu іm. V.N. Karazіna. Serіia: bіologіia. 2010. Vip. 11 (No. 905). P. 60–65. [in Ukrainian]

Bilynska O.V., Tymchuk S.M., Derebizova O.Yu. Artificial nutrient medium for barley haploid production in anther culture in vitro: Patent for invention 103426 Ukraine. No. a201210359; applied on 03.09.2012., published 10.10.2013, bulletin No. 19.

Bilynska O.V. Method of plant material pretreatment for spring barley haploid production in anther culture in vitro: Patent for invention 113261 Ukraine. No. a 2016 00611; applied on 21.05.2016., published 26.12.2016, bulletin No. 14.

Plokhinskii N.A. Biometriia. M.: Izd. Moskovskogo universiteta, 1964. 367 p. [in Russian]

Sunderland N., Huang B. Barley anther culture: the switch of programme and albinism. Hereditas. 1985. Supl. 3. P. 27−40.