Identification of agent of leaf spot desease of lupine based on the the syringomicin gene (syrD)

  • L. A. Dankevych
DOI: https://doi.org/10.7124/FEEO.v25.1151

Abstract

Aim. The detection of the phytotoxin syringomycin's secretion genes of (syrD) has been performed for the purpose of correct species and pathovar identification of isolated and collection strains of the agent of lupine's brown spottiness. Methods. It has been used microbiological and molecular genetic (PCR) methods. Results. The presence of phytotoxin syringomycin's secretion (syrD) genes in collection and isolated strains of the agent of lupine's brown spottiness and the typical strain Pseudomonas syringae pv. syringae UCM B-1027Т has been established. It has been shown that the amount of PCR product produced by collection strains "Pseudomonas lupini" varies and correlates with their pathogenic properties. Conclusions. Based on the results of the previous investigation and presented in this paper, the reclassification of the species "Pseudomonas lupini" finished and the strains previously belonging to this species were attributed to Pseudomonas syringae pv. syringae.

Keywords: identification, syringomycin's secretion genes (syrD), agent of lupine's brown spottiness.

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