Antiradiation properties of extracts from Hypericum perforatum L.

  • E. N. Shamilov
  • A. S. Abdullaev
  • V. E. Shamilli
  • I. V. Azizov
DOI: https://doi.org/10.7124/FEEO.v24.1121

Abstract

Aim. Considering the high biological activity of the components of the St. John’s wort, we conducted studies to identify its radioprotective effect. Methods. Studies were conducted on adult Vistar rats with an average weight of 100-120 grams.3 hours before irradiation, the extract from St. John’s wort was intraperitoneally administered to animals at a concentration of 50 mg/kg. Irradiation was carried out once with doses of 5 and 7 Gy on the “Rhund-20000” facility with an average radiation dose rate MD = 0.3069 Gr/s. The frequency of chromosomal abnormalities was recorded on the mitotic cells of the bone marrow. Results. Acute irradiation with a dose of 5 Gy led to cell division inhibition. The number of chromosomal aberrations increased due to structural disorders (deletions, fragments, inversions) up to 6.8%.In animals that received a dose of 7 Gy after a single irradiation, the frequency of chromosomal abnormalities increased to 11.02%. The introduction of the Hypericum perforatum L. extract softened the effect of ionizing radiation, reducing the mutational effect of radiation. It not only contributed to the restoration of the cell division, but also reduced the proportion of structural disorders. Conclusions. Hypericum extract can be considered to be promising as an anti-radiation agent of plant origin.

Keywords: rats, chromosomal abnormalities, marrow, irradiation, extract.

References

Yarmonenko S.P. Antiradiation protection of the body. M., 1969.

Aliev R.K., Prilipko L.I., Damirov I.A. Medicinal Plants of Azerbaijan; Directory. Baku: Azerbaijan State Publishing House, 1972.

Shamilov E.N., Abdullayev A.S., Rzayeva I.A., Azizov I.V. Influence of iron complexes on the formation of photosynthetic apparatus and yield of genetic changes in gamma-irradiated wheat seeds. Visnyk of Dnipropetrovsk University. Biology. Medecine. 2010. Vol. 18, No. 2. P. 90–95.

Azizov I.V., Abdullayev A.S., Shamilov E.N., Rzayev A.A., Akhmedov I.D. Effect of Hypericum extract (Hypericum perfora-tum L.) and peganum (Peganum harmala L.) on growth of wheat seedlings. "Proceedings of the Institute of Botany of NAS of Azerbaijan". Baku: "Elm", 2007. Vol. XXVII. P. 182–184.

Mamedov N.A., Rzayev A.A., Shamilov E.N., Abdullaev A.S., Craker L.E. Radioprotective activity of some medicinal plant extracts. Acta Hort. (ISHS). 2011. 925. P. 315–320. URL: http://www.actahort.org/books/925/925_46.htm (Last accessed: 5.03.2019).

Kasumov F.Y., Shamilov E.N., Abdullayev A.S., Kasumova N.I., Kuliev G.N. Investigation of the component composition of the extract of saffron (Crocus sativus L.) and its antiradiation action. Russia. Supplement to the magazine "Traditional Medi-cine". 2010. № 3 (22). P. 140–146.

Shamilov E.N., Abdullaev A.S., Akhmedov I.D., Azizov I.V. Study of antiradiation properties of polymer-modified extract of Hypericum perforatum L. Radiobiological and radioecological aspects of the Chernobyl disaster: abstracts of International Conference. Slavutych, 2011. P. 149.

Shamilov E.N. Comparative study of the impact of phytocomposition extract on some blood characteristics of irradiated rats. International Journal of Pharmaceutical Science and Health Care. 2015. Issue 5, Vol. 3. P. 118–121. URL: http://www.rspublication.com/ijphc/index.html ISSN 2249 – 5738 (Last accessed: 5.03.2019).

Shamilov E.N., Rzayev A.A., Abdullayev A.S., Kasumova N.I., Kuliev G.N., Mamedrzayeva E.T., Gaza A.G., Azizov I.V. Cytogenetic changes of irradiated rats under the influence of plant biocomplexes. Proceedings of the Georgian Academy of Sciences, Biological Series A. Tbilisi, 2009. Vol. 35. P. 393–401.

Azizov I., Shamilov E., Abdullayev A., Muslimova Z., Mamedli G., Gasimova G. Influence of a Modified Plant Extract on Activity of Antioxidant Enzymes and Concentration of Pigments in Gamma-Irradiated Plants of Maize and Wheat. Proceed-ings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 2018. Vol. 72, № 1. P. 38–42.

Ford C.E., Hamerton J.L. A colchicines hypotonic citrate squash sequence for mammalian chromosomes. Stain Technol. 1956. Vol. 31. P. 247–251.

Rajabli S.I., Kryukova E. The comparative analysis of the differential staining of chromosomes of two species of hamsters, Chinese and Daurian. Cytology. 1973. Vol. 15. P. 1527–1531.

Howell W.M., Dlack D.A. Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: in a one step method. Experientia. 1980. Vol. 36. P. 1014–1015.

Bochkov N.P., Kozlov V.M., Sevankaev A.V., Antoshina M.M. Analysis of aneuploidy in cultures of embryonic human fibro-blasts. Genetics. 1966. № 10. P. 120–124.

Zakharov A.F. Human chromosomes. Problems of linear organization. 1977. P. 2–192.