Polymorphism upstream the coding region of the Ppd-D1 gene in Aegilops tauschii Coss.

  • G. O. Chebotar
  • V. E. Sechnyak
  • T. Yu. Yurganova
  • S. V. Chebotar
DOI: https://doi.org/10.7124/FEEO.v23.1039

Abstract

Aim. Evaluate time of heading and flowering in Aegilops tauschii Coss. accessions, donor of  the bread wheat D genome, that are differentiated by polymorphism at upstream the coding region of the Ppd-D1 gene. Methods. NA extraction, allele-specific PCR, electrophoresis in polyacrylamide gel, two-factor analysis of variance. Results. We have found polymorphism upstream the coding region of Ppd-D1 gene with the help of the allele-specific PCR with primers to the Ppd-D1b allele in the accessions from the collection of Ae. tauschii Fragment 414 bp was detected in the samples: k-78, k-396, k-529, k-531, k-1144, k-1162, k-1235, k-1273, k-1283, fragment 429 bp have been revealed in the samples  k-187, k-968, k-2178, and 453 bp – in the samples k-80, k-2358, Tetra tauschii. The results of a three-year field experiment showed a significant effect of the detected polymorphisms on the time of heading and flowering. Conclusions. Among the Ae. tauschii accessions by allele-specific PCR with primers to the Ppd-D1b allele have been tested amplification fragments 414 bp, 429 bp and 453 bp. Allele Ppd-D1a was not found in the collection of Ae. tauschii accessions. Samples of aegilops that are carrier of insertions 24 + 15 bp (for which the fragment 453 bp was detected by allele-specific PCR) – were characterized by earlier heading and flowering time in certain years.

Keywords: time of heading and flowering, Ppd-D1 gene, Aegilops tauschiі Coss.

References

Totskii V.M., Dyachenko L.F., Muterko O.F., Balashova I.A., Toptikov V.A. Genetic determination and function of RR proteins, regulators of photoperiodic reactions, and circadian rhythms in plants. Cytol Genet. 2012. Vol. 46, No. 5. P. 319–334. doi: 10.3103/S009545271205009X.

Oleynikova T.V. Effect of day length and temperature on the formation of rudimentary spike in cereals. Morphogenesis of plants. Moscow: Izd. Moscow State University, 1961. Vol. 1. P. 133–170.

Langer S.M., Longin C.F.H., Würschum T. Flowering time control in European winter wheat. Front Plant Sci. 2014. Vol. 5. P. 537. doi: 10.3389/fpls.2014.00537.

Feldman M. Origin of cultivated wheat. In: Bonjean A.P., Angus W.J., editors. The world wheat book: a history of wheat breeding. Paris: Lavoisier Publishing, 2001. P. 3–56.

Guo Z., Song Y., Zhou R., Ren Z., Jia J. Discovery, evaluation and distribution of haplotypes of the wheat Ppd-D1 gene. New Phytologist. 2010. Vol. 185. P. 841–851. doi: 10.1111/j.1469-8137.2009.03099.x.

Fedorova V.R. Differences of the effects of photoperiodic sensitivity genes in winter bread wheat. PhD Thesis. Оdesa, 2004. 19 p.

Fayt V.I., Balashova I.A., Fedorova V.R., Balvinska M.S. Identification of bread wheat Ppd-genotypes by hybridological and STS-PCR analysis. Plant physiology and genetics. 2014. Vol. 46, No. 4. P. 325–336.

Beales J., Turner A., Griffiths S., et. al. A Pseudo-Response Regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theor. Appl. Genet. 2007. Vol. 115. P. 721–733. doi: 10.1007/s00122-007-0603-4.

Chebotar G.O., Chebotar S.V., Babenko D.O., Motsnyy I.I., Scherban A.B., Sivolap Yu. M. Alleles of Ppd-D1 gene in the collection of Aegilops tauschii accessions and bread wheat varieties. Biopolymers and Cell. 2012. Vol. 28, No. 2. P. 149–155. doi: 10.7124/bc.000042.

Bakuma A., Chebotar G., Filimonov V., Kyrylyuk T., Chebotar S. Haplotypes of Ppd-D1 gene and alleles of Ppd-A1 and Ppd-B1 in Ukrainian wheat varieties. Book of abstracts 4th Conference of Cereal Biotechnology and Breeding jointly organized by EUCARPIA Cereal Section (Budapest, 6–9 November, 2017). Cereal Research Communications 45 (Suppl.). 2017. P. 35–36. doi: 10.1556/0806.45.2017.100.

The using of PCR analysis in genetic-selection studies.Scientific and methodological guidance / Ed. Yu.M. Sivolap. Kyiv: Agrarna Nauka, 1998. P. 24–37.

Promega Technical Manual. Gene Print. STR Systems. New York, 1999. No. 7. 52 p.

Huang L. Wang Q., Zhang L., Yuan Z., Wang J., Zhang H., Zheng Y., Liu D. Haplotype variations of gene Ppd-D1 in Aegilops tauschii and their implications on wheat origin. Genet. Resour. Crop. Evol. 2011. Springer published online: doi: 10.1007/s10722-011-9741-2

Rokitsky P.F. Biological Statistics. Minsk: Vysheish. Shk., 1973. 320 p.