Actinomycetes of Juniperus excelsa Bield. rhizosphere – antagonists of phytopathogenic microbiota

  • S. I. Tistechok
  • V. Ya. Syrvatka
  • V. O. Fedorenko
  • O. M. Gromyko


Aim. Phytopathogenic microorganisms are one of the main causes of agricultural productivity losses. Thereby, the goal of this study was to evaluate actinomycetes strains, isolated from Juniperus excelsa Bield. rhizosphere, antagonistic activity against plant pathogenic bacteria and fungi. Methods. In this study we used microbiological methods for isolation actinomycetes from rhizosphere. Antagonistic activity was evaluated by using the dual culture method. Results. 372 actinomycete stains were isolated from J. excelsa Bield. rhizosphere. More than 60 % actinomyces isolates showed antibacterial activity against to lest one of the tested phytopathogenic bacteria genus Pseudomonas, Pectobacterium, Agrobacterium, Erwinia, Xanthomonas and 20.5 % of the tested phytopathogenic fungi genus Aspergillus, Alternaria, Fusarium, Botrytis. Only 2 strains had antagonistic activity to the all of the tested microorganisms and 62 strains, which had antagonistic activity to the one test-microorganism. Conclusions. Actinomicetes of J. excelsa Bield. rhizosphere are source for bioactive compounds against phytopatogenic microorganisms and showed good biotechnology potential. These results are the first step to the screening new biopesticides for controlling phytopatogenic diseases in plan.

Keywords: actynomicetes, phytopathogens, biocontrol.


Kaur T., Manhas R. K. Antifungal, insecticidal, and plant growth promoting potential of Streptomyces hydrogenans DH16. J. Basic Microbiol. 2013. Vol. 53. P. 1–13. doi: 10.1002/jobm.201300086.

Thind, T.S. Fungicide resistance: a perpetual challenge in disease control. J. Mycol. Plant Pathol. 2008. Vol. 38. P. 407–418.

Passari A.K., Mishra V.K., Gupta V.K., Yadav M.K., Saikia R., Singh B.P. In Vitro and In Vivo Plant Growth Promoting Activities and DNA Fingerprinting of Antagonistic Endophytic Actinomycetes Associates with Medicinal Plants. PLoS ONE. 2015. Vol. 10 (9). P. 1–18. doi: 10.1371/journal.pone.0139468.

Xue Q.Y., Ding G.C., Li S.M. et al. Rhizocompetence and antagonistic activity towards genetically diverse Ralstonia solanacearum strains – an improved strategy for selecting biocontrol agents. App Microbiol Biotechnol. 2013. Vol. 97. P. 1361–1371. doi: 10.1007/s00253-012-4021-4.

Goodfellow M., Williams S. Ecology of actinomycetes. Annu. Rev. Microbiol. 1983. Vol. 37. P. 189–216. doi: 10.1146/annurev.mi.37.100183.001201.

Red book of Ukraine. Y.P. Diduch. К.: Globalkonsalting, 2009. 900 p.

Gromyko O. Antifungal and antibacterial properties of actinomyces strains isolated from the rhizosphere of juniper Juniperus excelsa Bieb. Visnyk of Lviv Univ. Biology series. 2010. Vol. 53. P. 156–160.

Raju R., Gromyko O., Fedorenko V., Luzhetskyy A., Plaza A., Muller R. Juniperolide A: A New Polyketide Isolated from a Terrestrial Actinomycete, Streptomyces sp. Organic Lett. 2012. Vol. 14, Is. 23. P. 5860–5863. doi: 10.1021/ol302766z.

Raju R., Gromyko O., Fedorenko V., Luzhetskyy A., Muller R. Lorneic acids C and D, new trialkyl-substituted aromatic acids isolated from a terrestrial Streptomyces sp. J. of Antibiotics. 2013. Vol. 66. P. 347–349. doi: 10.1038/ja.2013.18.

Jiang Y., Li Q., Chen X., Jiang C. Isolation and Cultivation Methods of Actinobacteria. 2016. World's largest Science, Technology & Medicine. 2016. P. 39–57. doi: 10.5772/61457.

Kieser T., Bibb M., Chater K., Hopwood D. Practical Streptomyces genetics. Norwich: John Innes Foundation. 2000. P. 634.

Zenova H.M. Soil actinomycetes: book for study. Moskva. MGU, 1992. 79 p.

Hause H., Preobrashenskaia T., Sveshnikov M., Terehova L., Maksymova T. Determinant of actynomycetes. Genus Streptomyces. Moskva: Nauka, 1986. 245 p.

Zhang J. Improvement of an isolation medium for actinomycetes. Modern App. Sci. 2011. Vol. 5. P. 124–127.

Xie Q., Qu Z., Lin H-P., Li L., Hong K. Micromonospora haikouensis sp. nov., isolated from mangrove soil. Antonie van Leeuwenhoek. 2012. Vol. 101. P. 649 – 655. doi: 10.1007/s10482-011-9682-y.

Shirling E.B., Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Evol Bacteriol. 1966. Vol. 16. P. 312–340. doi: 10.1099/00207713-16-3-313.

Gromyko O., Tistechok S., Chornobai V., Fedorenko V. Antagonistic and plant growth promoting activities of rhizospheric actinomycetes from Thymus roegneri K. Koch Aggr. experim. evol. organ. 2017. Vol. 20. P. 129–133.

Balouiri M., Sadiki M., Ibnsouda S.K. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 2016. Vol. 6. P. 71–79. doi: 10.1016/j.jpha.2015.11.005.