Biochemical analysis and assessment of modern monogamous varieties of hemp Cannabis sativa L. by the content of cannabinoid substances
Aim. Defining the peculiarities of forming the cannabinoid substances in modern monogamous varieties of hemp is of both theoretical and practical importance. Methods. Modern monogamous varieties of hemp bred in Hlukhiv, Zolotonosha and specimens receive from France; were used for the chromatographically analysis of «damp» and dried in the shadow plant samples was conducted by the method of thin layer chromatography (TLC). Results. It was founding, that in the initial phase of growth and development the Ukrainian hemp varieties with the practical absence of neutral compounds and the French varieties with the sufficient content of these substances formed only natural acids. During the generative phase of growth and development, the varieties from France formed not only natural acids but also neutral compounds. The Ukrainian varieties demonstrated only insufficient increase of natural acids. Conclusions. In the initial phase of growth and development, the investigated hemp varieties containing both large and insignificant amount of cannabinoid substances formed only natural acids. Separate plants demonstrated the decrease of natural acids up to their total absence, which proves their biological interconnections and possible forming cannabidiol, tetrahydrocannabinol and сannabinol by way of fermentative decarboxylation the respective acids.
Keywords: Cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC), cannabidiolic acid (CBDA), tetrahydrocannabinolic acid (THCA).
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