Taxonomical position of pigmented yeast isolated from Antarctic ecosystems
Aim. To determine species composition and taxonomic position of extremotolerant Antarctic yeast. Methods. The objects of research were yeast from Antarctic soils and phytocenosis, which are stored in the Collection of Extremophilic Microorganisms at the Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiev. Yeast were grown on malt wort (рН 5.0–5.5, temperature 18–20°С). Isolation of genomic DNA was performed using a commercial kit DNA-sorb; amplification of DNA preparations – use of primers NL1 and NL4; phylogenetic analysis – construction of dendrograms showing the position of the studied strains among closely related and typical species. Results. The analysis have shown a high percentage of similarity (98.4–99.9 %) of 18S rRNA genes sequences of yeast Antarctic strains and yeast sequences from the database GenBank. It was found that yeast strains belonged to phylum Basidiomycota (genera Rhodosporidium, Rhodotorula) and Ascomycota (genus Exophiala). Conclusions. As it was shown by phylogenetic analysis, red yeast S33, S48, S182 clustered with Rhodotorula mucilaginosa; S14 – with Rhodosporidium diobovatum and can be attributed to these species. Extremotolerant black yeast S10, S36 and S237 refer to the species Exophiala nigra.
Keywords:Antarctica, extremotolerant yeast, phylogenetic analysis, species composition.
Carrasco M., Rozas J.M., Barahona S., Alcaíno J., Cifuentes V., Baeza M. Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. BMC Microbiol. 2012. Vol. 12. P. 251. doi: 10.1186/1471-2180-12-251.
Margesin R., Fonteyne P., Schinner F., Sampaio J.P. Rhodotorula psychrophila sp. nov., Rhodotorula psychrophenolica sp. nov. and Rhodotorula glacialis sp. nov., novel psychrophilic basidiomycetous yeast species isolated from alpine environments. Int. J. Syst. Evol. Microbiol. 2007. Vol. 57. P. 2179–2184. doi: 10.1099/ijs.0.65111-0
Vaz A.B.M., Rosa L.H., Vieira M.L.A., Garcia V., Brandão L.R., Teixeira L.C.R., Moliné M., Libkind D., van Broock M., Rosa C.A. The diversity, extracellular enzymatic activities and photoprotective compounds of yeasts isolated in Antarctica. Braz. J. Microbiol. 2011. Vol. 42. P. 937–947. doi: 10.1590/S1517-83822011000300012
Buzzini P., Branda E., Goretti M., Turchetti B. Psychrophilic yeasts from worldwide glacial habitats: diversity, adaptation strategies and biotechnological potential. FEMS Microbiol. Ecol. 2012. Vol. 82. P. 217–241. doi: 10.1111/j.1574-6941.2012.01348.x
Shivaji S., Prasad G.S. Antarctic yeasts: biodiversity and potential applications. In: Satyanarayana T., Kunze G. (eds) Yeast Biotechnology: Diversity and Applications. Springer-Verlag, Berlin. 2009. P. 3–16.
Connell L., Redman R., Craig S., Scorzetti G., Iszard M., Rodriguez R. Diversity of soil yeasts isolated from South Victoria Land, Antarctica. Microbial. Ecology. 2008. Vol. 56. P. 448–459. doi: 10.1007/s00248-008-9363-1.
Vasileva-Tonkova E., Romanovskaya V., Gladka G., Gouliamova D., Tomova I., Stoilova-Disheva M., Tashyrev O. Ecophysiological properties of cultivable heterotrophic bacteria and yeasts dominating in phytocenoses of Galindez Island, maritime Antarctica. World J. Microbiol. Biotechnol. 2014. Vol. 30. P. 1387–1398. doi: 10.1007/s11274-013-1555-2.
Laich F., Vaca I., Chávez R. Rhodotorula portillonensis sp. nov., a basidiomycetous yeast isolated from Antarctic shallow-water marine sediment. Int. J. Syst. Evol. Microbiol. 2013. Vol. 63. P. 3884–91. doi: 10.1099/ijs.0.052753-0.
Norshazliza Ab Ghani, Joanita Sulaiman, Zahidah Ismail, Xin-Yue Chan, Wai-Fong Yin. Rhodotorula mucilaginosa, a Quorum Quenching Yeast Exhibiting Lactonase Activity Isolated from a Tropical Shoreline. Sensors. 2014. Vol. 14. P. 6463–6473. doi: 10.3390/s140406463.
Romanovskaya V.A., Parfenova V.V., Belkova N.L., Sukhanova E.V., Gladka G.V., Tashirev A.B. Autecology, taxonomy, and strategy of surviving in extreme condition for antarctic microorganisms. Fundamental researches. 2014. Vol. 11 (9). P. 1954–1959.
Belkova N.L., Dzyuba E.V., Sukhanova E.V., Khanaeva T.A. Adaptation of methods of molecular genetic analysis for the study of microorganisms associated with fish. Biology of Inland Waters. 2008. Vol. 2. P. 91–94.
Anderson I.C., Cairney J.W. Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques. Environ. Microbiol. 2004. Vol. 6 (8). P. 769–779. doi: 10.1111/j.1462-2920.2004.00675.x
Belkova N.L. Molecular genetic methods for the analysis of microbial communities. In the book. Diversity of microbial communities in inland water reservoirs of Russia: Educational-methodical manual / ed. Andreeva A.M. Yaroslavl: pub. LLC «Print House», 2009. P. 53–63.
Romanovskaya V.A., Tashyrev A.B., Rokitko P.V., Shilin S.O., Chernaya N.A., Tashyreva A.O. Microbial diversity interrestrial Antarctic biotopes. Ukrainian Antarctic J. 2009. Vol. 8. P. 364–369.
Tashyrev A.B., Romanovskaya V.A., ShilinS.O., Chernaya N.A. Screening of a yeast-melanin producers in terrestrial Antarctic biotopes. Mikrobiol. Zh. 2010. Vol. 72. P. 3–10.
Issatschenko B.L. The study of bacteria of the Arctic Ocean. Petrograd: The printing house Kirschbaum V.O., 1914. P. 297.