PCR-analysis of photoperiod sensitivity genes in bread wheat varieties from Bilatserkovska experimental breeding station

  • V. M. Filimonov Odesa I. I. Mechnikov National University, Department of Genetics and Molecular Biology, Ukraine, 65082, Odesa, Dvoryanska str, 2
  • A. A. Bakuma Odesa I. I. Mechnikov National University, Department of Genetics and Molecular Biology, Ukraine, 65082, Odesa, Dvoryanska str, 2
  • G. A. Chebotar Odesa I. I. Mechnikov National University, Department of Genetics and Molecular Biology, Ukraine, 65082, Odesa, Dvoryanska str, 2
  • L. A. Burdenyuk-Tarasevich Bilotsekivs’ka Experimental Breeding Station, Ukraine, 09176, Kiev region, Belotserkovsky district, Malaya Olshanka, st. Sovetskaya, 1
  • S. V. Chebotar Odesa I. I. Mechnikov National University, Department of Genetics and Molecular Biology, Ukraine, 65082, Odesa, Dvoryanska str, 2; Plant Breeding and Genetics Institute — National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska doroga str., 3
DOI: https://doi.org/10.7124/visnyk.utgis.16.2.1060

Abstract

The aim. Determination of alleles of the photoperiod sensitivity genes Ppd-1 in 16 winter wheat varieties of
the Bilatserkovska Experimental Breeding Station and identification of the haplotypes of Ppd-D1 gene according to the accepted classification. Methods. DNA isolation, allele-specific and nested PCR, electrophoresis in agarose and polyacrylamide gels, determination of the significant differences in the time of heading. Results. Plants of the varieties Vodohrai bilotserkivs’kiy; Bilotserkivs’ka napivkarlykova, Olesia, Perlyna lisostepu, Elehiia, Yasochka, Lybid’, Tsarivna, Lisova pisnia, Romantyka, Vidrada, Schedra nyva, Charodiika bilotsekivs’ka, Russa, Driada 1 have the genotype PpdA1b Ppd-B1b Ppd-D1a and belong to the VII haplotype of the Ppd-D1 gene. The genotype of the variety Legenda bilotsekivs’ka is characterized by the presence of the alleles Ppd-A1b Ppd-B1b Ppd-D1b and belongs to the IV haplotype of the Ppd-D1 gene. The significant differences (P = 0.01) in the time of heading was detected only between the varieties Russa and Legenda bilotsekivs’ka and was 12.4 days. Conclusions. Most BEBS varieties are characterized by genotype — PpdA1b Ppd-B1b Ppd-D1a, in which the allele Ppd-D1a determines insensitivity to the photoperiod and leads to early heading. Only variety Legenda bilotsekivs’ka is the carrier of the recessive allele of Ppd-D1 gene, belongs to the IV haplotype, has the latest time of heading.
Keywords: Ppd-A1, Ppd-B1, Ppd-D1 genes, winter wheat, PCR analysis, photoperiod sensitivity.

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