Complex analysis of total protein content in bread wheat containing GPC-B1 gene from Triticum turgidum SSP. dicoccoides

  • S. Yu. Pokhylko Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnoho str., 148National Technical University of Ukraine«Igor Sikorsky Kyiv Polytechnic Institute»,Ukraine, 03056, Kyiv, Peromohy ave., 37
  • S. V. Schwartau Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • V. V. Pochynok Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • L. M. Mykhalska Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • O. M. Dugan National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Ukraine, 03056, Kyiv, Peromohy ave., 37
  • B. V. Morgun Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnoho str., 148Institute of Plant Physiology and Genetics, NationalAcademy of Sciences of UkraineUkraine, 03022, Kyiv, Vasylkivska str., 31/17
DOI: https://doi.org/10.7124/visnyk.utgis.15.1.712

Abstract

Aim. The purpose of our study was to determine the content of total protein in the F5 generation grains, the carriers of the Gpc-B1 gene from Triticum turgidum ssp. dicoccoides by two methods, which in turn would confirm the successful expression of the Gpc-B1 gene in the genetic environment of bread winter wheat. Methods. Determination of protein content was carried out by Kjeldahl method and by infrared spectrometry (NIR) method. Results. The 44 hybrid lines that are homozygous for the Gpc-B1 gene from T. turgidum ssp. dicoccoides have been analyzed. It has been established that for both methods, the average content of protein in the grain of hybrid lines is 14 % higher in comparison to the original Kuyalnik variety. Particular attention should be paid to the line number 10, 12 and 35 in which the content of protein exceeds 15 % by the method of Kjeldahl. Conclusions. The obtained results indicate that the gene Gpc-B1 from the wild relative in the new genetic environment of the highly productive registered wheat cultivar Kuyalnik has been functioning and has a positive effect on the accumulation of total protein in grains.

Keywords: biоfortification, protein content, Triticum aestivum, Gpc-B1 gene, Kjeldahl and NIR methods.

References

Zilic S., Barac M., Pesic M. et al. Characterization of proteins from grain of different bread and durum wheat genotypes. Int. J. Mol. Sci. 2011. Vol. 12(9). P. 5878–5894. doi: 10.3390/ijms12095878

Tarasyuk O.I., Pochinok V.M. Leaves nitrogen content and productivity of winter wheat lines unique in baking quality. Fiziol. rast. genet. 2015 Vol. 47(1). P. 66–73.

Atabaeva Kh. N., Massino I. V. Biologiia zernovykh kultur. Tashkent: Uzbekiston milliy entsiklopediiasi, 2005. P. 204.

Kasarda D. D., Bernardin J. E., Nimmo C. C. Wheat proteins. Advances in Cereal Science and Technology. (Y. Pomeranz, ed.). St. Paul, Minnesota: Am. Assoc. Cereal Chem., 1976. P. 158-236.

DSTU 3768–2010. Wheat. Technical Specifications. Derzhspozhyvstandart Ukrainy. 2010. P. 14.

Polianetska I. O., Liubych V. V., Sukhomud O. H. Vmist bilka ta yoho vykhid z urozhaiem zerna pshenytsi ozymoi zalezhno vid sortu. Naukovi pratsi instytutu bioenerhetychnykh kul'tur i tsukrovykh buriakiv. 2014. No 21. P. 235–239.

Simmonds N. The relation between yield and protein in cereal grain. Journal of the Science of Food and Agriculture. 1995. Vol. 67. P. 309–315. doi: 10.1002/jsfa.2740670306

Olmos S., Distelfeld A., Chicaiza O., Schlatter A. R., Fahima T., Echenique V., Dubcovsky J. Precise mapping of a locus affecting grain protein content in durum wheat. Theoretical and Applied Genetics. 2003. Vol. 107. P. 1243–1251. doi: 10.1007/s00122-003-1377-y

Joppa L. R., Du C., Hart G. E., Hareland G. A. Mapping gene(s) for grain protein in tetraploid wheat (Triticum turgidum L.) using a population of recombinant inbred chromosome lines. Crop Science. 1997. Vol. 37. P. 1586–1589. doi: 10.2135/cropsci1997.0011183X003700050030x

Uauy C., Brevis J. C., Dubcovsky J. The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat. J. Exp. Bot. 2006. Vol. 57. P. 2785–2794. doi: 10.1093/jxb/erl047

Tabbita F., Lewis S., Vouilloz J., Ortega M., Kade M., Abbate P., Barneix A. J. Effects of the Gpc-B1 locus on high grain protein content introgressed into Argentinean wheat germplasm. Plant Breeding. 2013. Vol. 132. P. 48–52. doi: 10.1111/pbr.12011

Pokhylko S.Yu., Troyanovska A.V., Stepanenko A.I., Urbanovich O.Yu., Dugan O.M., Rybalka O.I., Morhun B.V. Studies of bread wheat genotypes with transferred gene Gpc-B1 of Triticum turgidum ssp. dicoccoides. Faktori eksperimental'noi evolucii organizmiv. 2016. Vol. 18. P. 132–135.

GOST 10846-91 Zerno i produkty ego pererabotki. Metod opredeleniia belka Mezhgosudarstvennyy standart. 2009. P. 8.

DSTU 4117:2007 Grain and its products. Definition of quality indicators by infrared spectroscopy. Derzhspozhyvstandart Ukrainy, 2007 C. 8.

Student B. The probable error of a mean. Biometrika. 1908. Vol. 6(1). P. 1–25. Access mode: https://www.york.ac.uk/depts/maths/histstat/student.pdf