Spectral method of evaluation of photosynthetic apparatus thermal resistance

  • V. V. Shevchenko Institute of Plant Physiology and Genetics, NAS of Ukraine, 31/17 Vasylkivska St., Kyiv, 03022, Ukraine
  • O. Yu. Bondarenko Institute of Plant Physiology and Genetics, NAS of Ukraine, 31/17 Vasylkivska St., Kyiv, 03022, Ukraine
DOI: https://doi.org/10.7124/visnyk.utgis.14.2.694

Abstract

Aim. The aim of this work is to evaluate the possibilities of application of the spectral parameter - the relationship of the absorption values at wavelengths of 680 and 850 nm (k = A680 / A850) for testing heat resistance of plants to change the size of chloroplasts at short-term heating.  Methods. The short-term (5 minutes) heating in the range 25-45oC was carried out for chloroplasts isolated from two-week-old pea, corn and 4 winter wheat varieties differing in heat resistance. From the absorption spectra of chloroplasts was calculated changes of parameter k. Results. All heated chloroplasts showed changes in the spectral parameter k. Particularly strong and stable changes observed during heating at 40-45 ° C. In terms of changes in the spectral parameter plants are ranged in the following order - corn, pea, wheat. Conclusions. The intensity of the k changes coincided with the preliminary estimation of thermal stability for corn and different varieties of winter wheat. It is recommended to use the spectral parameter k for the rapid screening of varieties on the thermal stability of the photosynthetic apparatus within the same species.

Key words: chloroplasts of different species of plants, the photosynthetic apparatus, short-term heating, evaluation of the thermal stability.

References

Morgun V.V., Schwartau V.V., Kiriziy D.A. Physiological fundamentals of grain cereals high productivity. Physiology and Biochemistry of cultivated plants. 2010. Vol. 42, No. 5. P. 371-392.

Lobel D.V., Asner G.P. Climate and management contributions to recent trends in U.S. agricultural yields. Science. 2003. Vol. 299. P. 1032. doi: 10.1126/science.1077838

Berry J., Björkman O. Photosynthetic response and adaptation to temperature in higher plants. Ann.Rev. Plant. Physiol. 1980. Vol. 31. P. 491-543. doi: 10.1146/annurev.pp.31.060180.002423

Kreslavski V.D., Carpentier R., Klimov V.V., Murata N., Allakhverdiev S.I. Molecular mechanisms of stress resistance of photosynthetic apparatus. Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 2007. Vol. 1(3). P. 185–205.

doi: 10.1134/S1990747807030014

Kochubey S.M., Shevchenko V.V., Bondarenko O.Yu. Changes of pea chloroplast sizes induced by short-term heating. Physiology and Biochemistry of cultivated Plants. 2008. Vol. 40(2). P. 126-132.

Kochubey S.M., Shevchenko V.V., Bondarenko O.Yu. Dinamicheskie svoystva strukturnykh edinits khloroplastov: monograph. Kiev: Lohos, 2010. 176 p.

Shevchenko V.V., Bondarenko O.Yu. Changes of Chloroplast structure of pea leaves induced by short-term heating. Physiology and Biochemistry of cultivated Plants. 2012. Vol. 44(3). P. 265–269.

Morgun V.V., Sanin E.V., Schwartau V.V. Klub 100 tsentneriv. Suchasni sorty ta systemy zhyvlennia i zakhystu ozymoi pshenytsi: monograph. Kiev: Lohos, 2014. 152 p.

Kochubei, S.M., Volovik, O.I., Korneev, D.Yu., Porubleva, L.V., Shevchenko, V.V. Organization and functional activity of integral and granal fragments of pea thylakoids. Russ. J. Plant Physiol. 1998. Vol. 45(6). P. 695–701.

Bryant F.D., Latimer P., Seiber B.A. Changes in total light scattering and absorption caused by changes in particle conformation – A test of theory. Arch. Biochem. Biophys. 1969. Vol. 135(2). P. 109-117. doi: 10.1016/0003-9861(69)90521-9

Khlyust H. Rasseyanie sveta malymi chastitsami: monograph. Moskva: Myr, 1961. 358 p.

Bondarenko O.Yu. Changes of chloroplast sizes induced by short-term heating. Physiology and Biochemistry of cultivated Plants. 2010. Vol. 42(1). P. 79-83.

Bondarenko O.Yu. Changes of Pea chloroplast photosynthetic membranes under short-term heating. Physiology and Biochemistry of cultivated Plants. 2016. Vol. 48(1). P. 34-42.