The role of jasmonate signaling pathway in plant’s flowering genes response to ionizing radiation

  • M. V. Kryvokhyzha Institute of Cell Biology and Genetic Engineering NAS of Ukraine, 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine
  • K. V. Krutovsky Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Buesgenweg 2, 37077 Goettingen, Germany; Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, GSP-1, Moscow, Gubkina, 3, Russia; Genome Research and Education Center, Siberian Federal University, 660036, Krasnoyarsk, Akademgorodok, 50a, Russia; Department of Ecosystem Science and Management, Texas University, College Station, TX, USA
  • N. M. Rashydov Institute of Cell Biology and Genetic Engineering NAS of Ukraine, 148 Academika Zabolotnoho St., 03143, Kyiv, Ukraine
DOI: https://doi.org/10.7124/visnyk.utgis.17.1.1200

Abstract

Aim. This study aimed to characterize the role of the jasmonate signaling pathway in flowering genes response to acute and chronic ionizing irradiation in plants. Methods. We used the wild-type Arabidopsis thaliana and jasmonic pathway defective jin mutant of Col0 ecotype in our experiments. The chronic irradiation was provided by 137СsCl with a total dose of 17 cGy and a dose rate of 6.8×10-6 cGy/s. The acute irradiation experiment was performed on 21 days old plants at the 5.0 stage (Boyes 2001) by X-rays in a total dose of 5 Gy with a dose rate of 89 cGy/s. The length of stems and leaves was measured in post-irradiation period. The molecular genetic analysis was done using real-time PCR. We determined the relative expression of key flowering genes AP1, GI, FT, CO, ACT2 with UBQ10 used as reference genes. Statistical analysis of phenotypic parameters was done using Student’s t-test in GraphPad Prism 8 software. The quantitative PCR data were analyzed in the REST 2009 software, QIAGEN. Results. The plant groups differed significantly by the stem length (p>0,05). The study revealed decreased expression of CO, GI and FT genes in jin mutants. The overexpression of AP1 in jin mutants under chronic irradiation may cause cell division errors and impact flower development. In contrast, AP1 expression in WT plants was near to normal =1 under chronic irradiation. These results suggest the involvement of the jasmonate pathway in the regulation of plants flowering during the irradiation. Сonclusion. Based on the results of our study, we hypothesize that jasmonic acid has a stabilizing effect on the rate of cell differentiation in plants under chronic irradiation. Despite the uncovered role of jasmonic acid in Arabidopsis thaliana flowering the exact mechanism of its action remains unclear and requires further investigation.
Keywords: jasmonate signaling, jasmonic acid, JA, flowering, ionizing radiation, real-time PCR, relative expression.

References

Boyes D.C. Growth Stage-Based Phenotypic Analysis of Arabidopsis: A Model for High Throughput Functional Genomics in Plants. The plant cell online. 2001. Vol. 13(7). P. 1499.

Caplin N., Willey N. Ionizing radiation, higher plants, and radioprotection: from acute high doses to chronic low doses. Front Plant Sci. 2018. No 9. P. 847. doi: 10.3389/fpls.2018.00847

Dmitriev O. P., Kravets O. P., Rashydov N. M., and others. Epigenetic factors of plant adaptation. Kyiv: PALIVODA A.V., 2018. 284 p.

Gregis V., Sessa A., Colombo L., Kater M.M. AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control AGAMOUS during early stages of flower development in Arabidopsis. Plant Cell. 2006. Vol. 18. P.1373-1382. doi: 10.1105/tpc.106.041798

Hong M.J., Kim D.Y., Ahn J.W., Kang S.Y., Seo Y.W. Kim J.B. Comparison of radiosensitivity response to acute and chronic gamma irradiation in colored wheat. Genet. Mol. Biol. 2018. Vol. 41(3). Epub. doi: 10.1590/1678-4685-gmb-2017-0189

Karpets Y.V., Kolupaev Y.E., Lugovaya A.A., Oboznyi A.I. Effect of jasmonic acid on the pro-/antioxidant system of wheat coleoptiles as related to hyperthermia tolerance. Russian Journal of Plant Physiology. 2014. Vol. 61(3). P. 339-346.

Kolupaev Y.E., Karpets Y.V. Jasmonic acid in plants: synthesis, signaling and physiological effects in stress. Bulletin Of Kharkiv National Agrarian University. Biology series. 2010. Is. 1, No. 19. P. 21-33.

Kryvokhyzha M.V., Krutovsky K.V., Rashydov N.M. Differential expression of flowering genes in Arabidopsis thaliana under chronic and acute ionizing radiation. International Journal of Radiation Biology. 2019. Epub. doi: 10.1080/09553002.2019.1562251

Lugovoy A.A., Karpets Y.V., Oboznyi A.I., Kolupaev Y.E. Stress-protective effect of jasmonic and succinic acids on barley plants in soil drought conditions. Agrochemistry. 2014. No. 4. P. 48-55

Nesterenko L., Rashydov N. The Problems Sustainable Remediation Of The Chernobyl Alienation Areas. Journal of Radiation Researches. 2018. Vol. 5(2). P.13-25

Pfaffl M, Horgan G, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research. 2002. Vol. 30(9). P. e36. doi:10.1093/nar/30.9.e36

Rashydov N., Kliuchnikov O., Seniuk O. et al. Radiobiological characterization environment around object “Shelter”. Chapter 7 in Nuclear Power Plant. edit. by Soon Heung Chang, 2012. P. 231-279. doi: 10.13140/2.1.2519.1360

REST 2009 Software User Guide, QIAGEN GmbH. 2009. 28 p.

Ruan J., Zhou M., Yan J., Khurshid M., Weng W., Cheng J., Zhang K. Jasmonic acid signaling pathway in plants. Int. J. Mol. Sci. 2019. Vol. 20, No. 10. P. 2479; doi: 10.3390/ijms20102479.

Volkov R.A., Panchuk I.I., Schöffl F. Heat-stress-dependency and developmental modulation of gene expression: the potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCR. J. Experiment. Bot. 2003. V.54. P. 2343-2349.

Yastreb T.O., Kolupaev Y.E., Lugovaya A. A., Dmitriev A. P. The contents of osmolytes and flavonoids of Arabidopsis thaliana defective in jasmonate signaling under salt stress. Applied Biochemistry and Microbiology. 2016. Vol. 52(2). P. 223-229. doi: 10.7868/S0555109916020185