Polymorphism in the short arm of 1R rye chromosomes in wheat lines with 1RS.1BL translocation and 1R(1B) substitution from different sources

  • M. K. Toporash Odessa I.I. Mechnikov National University, Ukraine, 65082, Odessa, Dvoryanskaya str., 2
  • I. I. Motsnyy Plant Breeding and Genetics Institute —National Center of Seed and Cultivar Investigations, Ukraine, 65036, Odessa, Ovidiopolskaya dor., 3
  • A. Börner Leibniz Institute of Plant Genetics and Crop Plant Research, Germany, D-06466, Gatersleben, Corrensstrasse, 3
  • P. Sourdille UMR 1095 INRA-UBP Génétique, Diversité & Ecophysiologie des Céréales, France, 63039, Clermont-Ferrand, Chemin de Beaulieu, 5
  • S. V. Chebotar Odessa I.I. Mechnikov National University, Ukraine, 65082, Odessa, Dvoryanskaya str., 2; Plant Breeding and Genetics Institute —National Center of Seed and Cultivar InvestigationsUkraine, 65036, Odessa, Ovidiopolskaya dor., 3
DOI: https://doi.org/10.7124/visnyk.utgis.16.2.1059

Abstract

Aim. The short arm of 1R rye (Secale cereale L.) chromosome is widely used in the breeding of bread wheat (Triticum aestivum L.), in particular 1RS.1BL, to introsgress genes of resistance to leaf (Lr26), stem (Sr31), striped (Yr9) rusts, as well as powdery mildew (Pm8); 1RS.1AL carries Gb2/Gb6 resistance genes to the wheat aphid (Schizaphis graminum Rondani), powdery mildew (Pm17), and the Cmc4 resistance gene to the Aceria tosichella Koifer mite, which is a vector for spreading of wheat mosaic virus. The aim of the research is to reveal molecular genetic polymorphisms of short arm rye 1RS chromosomes of different origins in bread wheat lines with 1RS.1BL translocation or 1R(1B) substitution from different sources. Methods. Genetic polymorphism of lines was analyzed by using PCR with a number of rye and wheat microsatellite markers. Results. It was shown that the CWXs line has a recombinant 1RS arm that contains the chromosomes parts of 1RS of the parental lines H242/97-2 and H273/97, due to crossover event, which led to the recombination of marked loci. Conclusions. Molecular genetic polymorphism has been reviled in 1RS.1BL translocations and 1R substituted rye chromosomes of different origins in H242/97-2, CWXs, H273/97, PavonMA1, Salmon lines, as there are different alleles present at loci: Xscm9, Xtsm422, Xgwm752,
Xgwm18, Taglgap.
Keywords: polymorphism, 1RS.1BL translocation, PCR analysis, microsatellites markers.

References

Crespo-Herrera L. A., Garkava-Gustavsson L., Ahman I. A systematic review of rye (Secale cereale L.) as a source of resistance to pathogens and pests in wheat (Triticum aestivum L.). Hereditas. 2017. Vol. 154(14). P. 14–23. doi: 10.1186/s41065-017-0033-5

Devos K.M., Bryan G.J., Collins A.J., Stephenson P., Gale M.D. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theoretical and Applied Genetics. 1995. Vol. 90(2). P. 247–252.

Dhaliwal, A. S., and MacRitchie F. Contributions of protein fractions to dough handling properties of wheat-rye translocation cultivars. J. Cereal Sci. 1990.Vol. 12(2). P. 113–122. doi: 10.1016/S0733-5210(09)80093-3

Doyle J.J., Doyle J.L. Isolation of plant DNA from fresh tissue. Focus. 1990. Vol. 12(1). P. 13–15.

Graybosch R.A. Uneasy unions: Quality effects of rye chromatin transfers to wheat. J. Cereal Sci. 2001. Vol. 33(1). P. 3–16. doi: l0.1006/jcrs.2000.0336

Howell T., Hale I., Jankuloski L., Bonafede M., Gilbert M., Dubcovsky J. Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status. Theoretical and Applied Genetics. 2014. Vol. 127(12). P. 695–2709. doi: 10.1007/s00122-014-2408-6

Karki D., Wyant III W., Berzonsky W.A., Glover K.D. Investigating physiological and morphological mechanisms of drought tolerance in wheat (Triticum aestivum L.) lines with 1RS translocation. Am J Plant Sci. 2014. Vol. 5(13). P. 1936–1944. doi: 10.4236/ajps.2014.513207

Kattermann G. Über konstante, halmbehaarte stämmeaus weizenroggenbastardierung mit 2n=42 chromosomen. Z. Indukt. Abstamm. Vererbungsl. 1938. Vol.74. P. 354–375.

Khlestkina E.K., Than M. H. M., Pestsova E. G., Röder M. S., Malyshev S. V., Korzun V., Börner A. Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theoretical and Applied Genetics. 2004. Vol. 109(4). P. 725-732. doi: 10.1007/s00122-004-1659-z

Kofler R., Bartos J., Gong L., Stift G., Suchankova P., Simkova H., Berenyi M., Burg K., Dolezel J., Lelley T. Development of microsatellite markers specific for the short arm of rye (Secal cereale L.) chromosome 1. Theoretical and Applied Genetics. 2008. Vol. 117(6). P. 915–926. doi: 10.1007/s00122-008-0831-2

Kozub N.A., Sozinov I.A., Karelov A.V., Bidnyk H.Ya., Demianova N.A., Sozinova O.I., Blume Ya.B., Sozinov A.A. Studying recombination between the 1RS arms from the rye Petkus and Insave involved in the 1BL.1RS and 1AL.1RS translocations using storage protein loci as genetic markers. Cytology and Genetics. 2018. Vol. 52(6). P. 440–447. doi: 10.3103/S0095452718060063

Lein, A. Introgression of a rye chromosome to wheat strains by Georg Riebesel - Salzmünde after 1926. Proc. EUCARPIA Symp. on Triticale, Leningrad. 1973. P.158-167

Lukaszewski AJ. Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Sci. 2000. Vol. 40(1). P. 216–225. doi: 10.2135/cropsci2000.401216x

Mago R., Spielmeyer W., Lawrence G.J., Lagudah E.S., Ellis J.G., Pryor A. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theoretical and Applied Genetics. 2002. Vol.104(8). P. 1317–1324. DOI: 10.1007/s00122-002-0879-3

Merker A., Forsstrom P. Isolation of mildew resistant wheat-rye translocation lines from a double substitution line. Euphytica. 2000. Vol. 15(3). P. 167–172. doi: 10.1023/A:1004018500970

Rabinovich S.V. Importance of wheat-rye translocations for breeding modern cultivar of Triticum aestivum L. Euphytica. 1998. Vol. 100(1). P. 323–340.

Röder M. S., Wendehake K., Korzun V., Bredemeijer G., Laborie D., Bertrand L., Isaac P., Rendell S., Jackson J., Cooke R. J., Vosman B., Ganal M. W. Construction and analysis of a microsatellite-based database of European wheat varieties. Theoretical and Applied Genetics. 2002. Vol. 106(1). P. 67–73. doi: 10.1007/s00122-002-1061-7

Röder M.S. Korzun V., Wendehake K., Plaschke J., Tixier M., Philippe L., and Ganal M. W. A microsatellite map of wheat. Genetics. 1998. Vol. 149(4). P. 2007-2023.

Saal B. and Wricke G. Development of simple sequence repeat markers in rye (Secale cereale L.) Genome. 1999. Vol.42(5). P. 964–972.

Schlegel R. Current list of wheats with rye and alien introgression. 2016. V05-16, 1-18. URL: http://www.rye-gene-map.de/rye-introgression

Sebesta E.E., Wood E.A., Porter D.R., Webster J.A., Smith E.L. Registration of gaucho greenbug-resistant triticale germplasm. Crop Sci. 1994. Vol. 34(5). P. 1428–1428.

Tsunewaki K. Genetic studies of a 6x-derivative from an 8x Triticale. Canad. J. Genet. Cytol. 1964. Vol. 6(1). P. 1–11.

Vosman B., Cooke R., Ganal M., Peeters R., Isaac P., Bredemeijer G. Standardization and application of microsatellite markers for variety identification in tomato and wheat. Acta Hort. 2001. Vol. 546. P. 307–316.

Weng Y., Azhaguvel P., Devkota R. N. and Rudd J. C. PCR-based markers for detection of different sources of 1AL.1RS and 1BL.1RS wheat–rye translocations in wheat background. Plant Breeding. 2007. Vol. 126(5). P.482–486. doi: 10.1111/j.1439-0523.2007.01331.x

Zarco-Hernandez J.A., Santiveri F., Michelena A., Javier Peña R. Durum wheat (Triticum turgidum, L.) carrying the 1BL/1RS chromosomal translocation: agronomic performance and quality characteristics under Mediterranean conditions. Eur J. Agron. 2005. Vol. 22(1). P.33–43. doi: 10.1016/j.eja.2003.12.001