Antiproliferative potential of mouse embryonic fibroblasts secreting IFN-β or IL-21, upon cocultivating with Lewis lung adenocarcinoma cells

  • I. N. Vagyna Institute of Molecular Biology and GeneticsNAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo str., 150
  • O. A. Zaharuk Institute of Molecular Biology and Genetics NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo Str., 150
  • L. I. Strokovska Institute of Molecular Biology and Genetics NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo Str., 150
  • Yu. V. Vagyn Institute of Molecular Biology and Genetics NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo Str., 150
  • V. I. Kashuba Institute of Molecular Biology and Genetics NAS of Ukraine, Ukraine, 03143, Kyiv, Akademika Zabolotnogo Str., 150
DOI: https://doi.org/10.7124/visnyk.utgis.15.1.705

Abstract

Aim. Investigation of the effect of mouse embryonic fibroblasts (C57Fb), transduced with baculovirus vectors (BVs), producing IFN-β and IL-21 cytokines on survival and proliferation of Lewis lung adenocarcinoma cells (LL). Methods. Construction of BVs, transduction of cells, fluorescence microscopy, flow cytometry. Results. It was shown that adenocarcinoma cells were more sensitive to the antiproliferative effect of IFN-β and IL-21. The efficacy of inhibiting the proliferation of tumor cells LL was higher when co-cultured heterologous cells C57Fb/IFNβ: LL. Cocultivation of C57Fb cells loaded with the BV-IL21 vector and cells LL caused a slight inhibition of adenocarcinoma cell proliferation. The mouse embryonic fibroblasts suppressed proliferation of cells LL upon co-cultivating. Conclusions. Interferon β synthesized by mouse embryonic fibroblasts or tumor cells LL, that were transduced with BVs carrying mouse Ifn-β gene, inhibited proliferation of adenocarcinoma malignant cells in vitro. Interleukin-21, produced by transduced tumor cells LL, effectively inhibited the proliferation of these cells.

Keywords: interferon-β (IFN-β), interleukin-21 (IL-21), mouse embryonic fibroblasts (C57Fb), lung adenocarcinoma cell line (LL), baculovirus vector (BV).

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