The content of polyphenolic compounds in the Arabidopsis thaliana knockout mutant Cat2 under salt stress

  • N. O. Didenko Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • I. M. Buzduga Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • R. A. Volkov Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • I. I. Panchuk Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
DOI: https://doi.org/10.7124/visnyk.utgis.14.2.683

Abstract

Aim. In plants, the defense response to abiotic stress includes induction of stress proteins and increase in content of protective metabolites. To date, the role of specific isoforms of antioxidant enzymes in stress responses and their relation to low-molecular weight protective compounds are still not clarified. To study this question the content of polyphenolic compounds (PPС) was evaluated under salt stress in Arabidopsis thaliana wild-type (WT) and in catalase 2 (Cat2) knockout mutant plants. Methods. PPС content in different variants of treatment with sodium chloride was measured. Results. It was shown that under optimal cultivation conditions the content of PPS in leaves of cat2 mutants is higher than in WT leaves. However, cultivation of isolated shoots in nutrient medium resulted in a faster depletion of the PPС pool in the cat2 line. Also, short-term salt stress results in equal depletion of the PPС pool in both, WT and cat2. Conclusions. The increase of PPС content in cat2 leaves is a manifestation of metabolic alterations that aim to compensate the reduced catalase activity.

Keywords: multigenic family, knockout mutants, polyphenolic compounds, sodium chloride, Arabidopsis thaliana.

References

Kim Y. H., Kim C. Y., Lee H. S., Kwak S. S. Changes in activities of antioxidant enzymes and their gene expression during leaf development of sweet potato. Plant Growth Regulation. 2009. Vol. 58(3). P. 235–241. doi: 10.1007/s10725-009-9371-2

Orendi G. Expression von Katalasen während der Blattseneszens und unter verschiedenen Stressbedingungen in Arabidopsis thaliana (L.) Heynh.: Dissertation Verlag Grauer, 2001. 135 p.

Panchuk I. I., Zentgraf U., Volkov R. A. Expression of the Apx gene family during leaf senescence of Arabidopsis thaliana. Planta. 2005. Vol. 222(5). P. 926–932. doi: 10.1007/s00425-005-0028-8

Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish Journal of Environmental Studies. 2006. Vol. 15(4). P. 523–530.

Ahmad P., Jaleel C. A., Salem M. A., Nabi G., Sharma S. Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Journal Critical Reviews in Biotechnology. 2010. Vol. 30(3). P. 161–175. doi: 10.3109/07388550903524243

Sakihama Y., Cohen M. F., Grace S. C., Yamasaki H. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology. 2002. Vol. 177(1). P. 67–80. doi: 10.1016/S0300-483X(02)00196-8

Hasegawa P. M., Bressan R. A., Zhu J. K., Bohnert H. J. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology. 2000. Vol. 51(1). P. 463–499. doi: 10.1146/annurev.arplant.51.1.463

Sneha S., Rishi A., Chandra S. Effect of short term salt stress on chlorophyll content, protein and activities of catalase and ascorbate peroxidase enzymes in Pearl millet. American Journal of Plant Physiology. 2013. Vol. 9(1). P. 32–37. doi: 10.3923/ajpp.2014.32.37

Deinlein U., Stephan A. B., Horie T., Luo W., Xu G., Schroeder J. I. Plant salt-tolerance mechanisms. Trends in Plant Science. 2014. Vol. 19(6). P. 371–379. doi: 10.1016/j.tplants.2014.02.001

Falleh H., Jalleli I., Ksouri R., Boulaaba M., Guyot S., Magné C., Abdelly C. Effect of salt treatment on phenolic compounds and antioxidant activity of two Mesembryanthemum edule provenances. Plant Physiology and Biochemistry. 2012. Vol. 52. P. 1–8. doi: 10.1016/j.plaphy.2011.11.001

Budzak V. V. Biometrics. Chernivtsi: Ruta, 2013. 326 p.

Doliba I. M., Volkov R. A., Panchuk I. I. Activity of catalase and ascorbate peroxidase in Cat2 knock-out mutant of Arabidopsis thaliana upon cadmium stress. Visn. Ukr. Tov. Genet. Sel. 2011. Vol. 9(2). P. 200–209.

Didenko N. O., Buzduga I. M., Volkov R. A., Panchuk I. I. The influence of acute salt stress on the lipid peroxidation in Arabidopsis thaliana. Buletin stiintific Chisinau. 2015. Vol. 22(35). P. 16–20.

Parida A., Das A. B., Das P. NaCl stress causes changes in photosynthetic pigments, proteins, and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures. Journal of Plant Biology. 2002. Vol. 45(1). P. 28–36. doi: 10.1007/BF03030429

Ksouri R., Megdiche W., Debez A., Falleh H., Grignon C., Abdelly C. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiology and Biochemistry. 2007. Vol. 45(3). P. 244–249. doi: 10.1016/j.plaphy.2007.02.001

Abdallah S. B., Rabhi M., Harbaoui F., Zarkalai F., Lachaal M., Karray-Bouraoui N. Distribution of phenolic compounds and antioxidant activity between young and old leaves of Carthamus tinctorius L. and their induction by salt stress. Acta Physiologia Plantarum. 2013. Vol. 35(4). P. 1161–1169. doi: 10.1007/s11738-012-1155-z