Характеристика сортів пшениці селекції Полтавської державної аграрної академії за допомогою маркерів до генів, що визначають важливі господарсько-агрономічні ознаки
Анотація
Метою роботи було визначення алелів генів Ppd-А1, Ppd-В1, Ppd-D1, Rht8, Rht-B1, Rht-D1, Pina-D1, Pinb-D1, Wx-A1, Wx-B1, Wx-D1 у сортів озимої м’якої пшениці, створених в Полтавській державній аграрній академії (ПДАА). Методи: виділення ДНК, алель-специфічна та SSRПЛР, електрофорез в агарозному та поліакриламідному гелях. Результати. Переважну більшість сортів (9) можна охарактеризувати як генотипи з алелями: Ppd-А1b, Ppd-В1b, PpdD1a, Rht8c, Rht-B1a, Rht-D1a, Pina-D1a, Pinb-D1b, Wx-A1a, Wx-B1a, Wx-D1a. В той же час сорти Левада, Царичанка, Лютенька і Оржиця є носіями Ppd-А1b, Ppd-В1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1b, Pina-D1a, Pinb-D1b, Wx-A1a, Wx-B1a, Wx-D1a алелів, а сорт Сидор Ковпак (Ppd-А1b, Ppd-В1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1a, Pina-D1a, Pinb-D1а/b, Wx-A1a, Wx-B1a/b, Wx-D1a) виявився гетерогенним за генами, що відповідають за якість зерна. Висновки. Сорти пшениці озимої селекції Полтавської державної аграрної академії характеризуються високим рівнем однорідності за алелями генів, що визначають важливі господарсько-агрономічні ознаки, що може бути обумовлено потребами певної зони вирощування та традиційними підходами до селекції цієї культури у ПДАА.
Ключові слова: Алель-специфічні маркери; озима м’яка пшениця; гени короткостебловості; чутливості до фотоперіоду; Wx; Pina та Pinb.
Посилання
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