Allelic state of the molecular genetic markers for genes associated with sensitivity to Pyrenophora tritici-repentis toxins A and B and Staganospora nodorum toxin A among Ukrainian common wheat cultivars

  • A. V. Karelov Institute of Plant Protection, NAAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2a
  • N. A. Kozub Institute of Plant Protection, NAAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2a
  • I. A. Sozinov Institute of Plant Protection, NAAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 33
  • A. A. Sozinov Institute of Plant Protection, NAAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska str., 33; Institute of Food Biotechnology and Genomics, NAAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2a
  • Ya. B. Blume Institute of Food Biotechnology and Genomics, NAAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2a

Abstract

Aim. The study was performed to characterize wheat cultivars of Ukrainian breeding using XBE44541, Xfcp394 and Xfcp623 molecular genetic markers for the Tsn1 and Tsc2 genes conferring sensitivity to the toxins A of the necrotrophic fungi P. tritici-repentis and S. nodorum and toxin B of P.tritici-repentis. Methods. PCR with primers flanking diagnostic markers of the genes and DNA samples of 160 common wheat (T. aestivum L.) cultivars of Ukrainian breeding was used. Results. The frequency of the marker Xfcp394 allele for ToxA insensitivity was extremely high and made up 96.3 %. The frequency of the marker Xfcp623 allele for ToxA insensitivity was lower and made up 60.6 %. The accuracy of the marker Xfcp394 with respect to marker Xfcp623 made up near 59 %. The total amount of the marker XBE444541 for ToxB insensitivity made up 66.8 %. Conclusion. The alleles for the ToxA and ToxB insensitivity of the Tsn1 and Tsc2 genes are common among Ukrainian wheat cultivars and may be used for pyramiding in breeding process.

Keywords: common wheat, tan spot, S. nodorum blotch, molecular genetic markers.

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