Frequency of genetic factors of HIV/AIDS resistance among people from Western region of Ukraine

  • М. Я. Тиркус Institute of Hereditary Pathology of the Ukrainian National Academy of Medical Sciences Ukraine, 79000, Lviv, Lysenko str., 31-a
  • Г. В. Макух Institute of Hereditary Pathology of the Ukrainian National Academy of Medical Sciences Ukraine, 79000, Lviv, Lysenko str., 31-a
  • І. М. Дмитрук Institute of Hereditary Pathology of the Ukrainian National Academy of Medical Sciences Ukraine, 79000, Lviv, Lysenko str., 31-a
  • Г. Р. Акопян Institute of Hereditary Pathology of the Ukrainian National Academy of Medical Sciences Ukraine, 79000, Lviv, Lysenko str., 31-a

Abstract

Aim. To study the frequency of allelic variants of human chemokine receptors genes which lead to higher resistance/sensibility to HIV-1 among Western Ukrainian population. Methods. DNA from peripheral blood leukocytes was isolated and purified using a modified salting out method. Extracted DNA was amplified by Polymerase chain reaction (PCR). The PCR products were subsequently digested with the restriction enzyme for identification of chemokine receptor genes mutations. The PCR products were subjected to electrophoresis in 2 % agarose gel. Results. A molecular genetic study of chemokine receptor gene was performed and frequency of CCR5del32 mutation was established among people from Western Ukrainian population. 17.0 % of inhabitants are heterozygous and 0,5 % are homozygous carriers of CCR5del32 mutation that is higher than in other European populations. The CCR 2-64I mutation was revealed in 14,5 % of people from Western Ukraine. It is the same percentage as in other studied ethnic European groups. Нomozygous mutation CCR2-64I was revealed in 1,5 % people. Heterozygous mutation SDF-1 3'A of CXCR-4 gene was found in 30.5 % and homozygous in 3 % persons of studied group. Nineteen compound heterozygotes for mutations of chemokine receptor genes were identified among studied group of Western Ukraine population. The number of people who do not have any protective genotype is 33 %. Conclusions.The obtained results on the frequency of chemokine receptors gene mutations among Western Ukrainian population indicate their higher genetic resistance to HIV-1 infection compared with other ethnic groups.

Key words: HIV infection, chemokine receptor, mutation, resistance.