Estimation of genetic diversity for different species of woody plants by intron length polymorphism of beta-tubulin genes

  • L. O. Kalafat Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
  • N. N. Pirko Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
  • A. Ye. Demkovych Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
  • S. N. Privalikhin Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
  • A. N. Rabokon Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
  • Ya. V. Pirko Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
  • Ya. B. Blume Institute of Food Biotechnologyand Genomics NAS of Ukraine, Ukraine, 04123, Kyiv, Osipovskogo str., 2A
DOI: https://doi.org/10.7124/visnyk.utgis.15.2.874

Abstract

Aim. The effectiveness of using the method of intron length polymorphism of β-tubulin genes is analyzed to evaluate the genetic diversity and polymorphism of tree plants by approbating it for a broad sample of tree plants of different taxonomic positions. Method. The method of estimation intron length polymorphism of β- tubulin (tubulin base polymorphism — TBP-method) has been tested. Results. The molecular genetic profiles and the unique patterns for the Quercus robur L., Populus tremula L., Fagus sylvatica L., Fagus sylvatica f. salicifolia, Robinia pseudoacacia L., Morus alba L., Ulmus glabra Huds., Betula pendula Roth., Acer platanoides L., Acer negundo L., Acer saccharinum Marshall, Catalpa bignonioides Walter, Tilia cordata Mill., Tilia platyphyllos Scop., Aesculus hippocastanum L., Populus nigra L., Juglans regia L., Fraxinus excelsior L., Alnus glutinosa (L.) Gaertn., Ginkgo biloba L. have been created. Some common fragments inherent in individual genera within the family have been found. Conclusions. TBP-method is rather convenient and reliable. It can be used both for molecular genetic marking and for the study of intra- and interspecific polymorphism of economically valuable, horticultural and forest trees.

Keywords: TBP-method, β-tubulin introns, tree plants, genetic diversity.

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