Expression of cyanobacterium desC transgene does not change canola plant cold resistance

  • М. С. Сливец Institute of Cell Biology and Genetic Engineering NAS of Ukraine Ukraine, 03680, Kyiv, Zabolotnoho str., 148 National Technical University of Ukraine “Kyiv Polytechnic Institute”, Ukraine, Kyiv, 03056, Peremogy prospect, 37
  • Л. А. Сахно Institute of Cell Biology and Genetic Engineering NAS of Ukraine Ukraine, 03680, Kyiv, Zabolotnoho str., 148
  • Ю. В. Шелудько Institute of Cell Biology and Genetic Engineering NAS of Ukraine Ukraine, 03680, Kyiv, Zabolotnoho str., 148

Abstract

Aim. Canola plants which were obtained earlier expressing Synechococcus vulcanus desС transgene growth tеsting under low positive temperatures in aseptic culture. Target gene was fused with reporter licBM3 (thermostable lichenase) of Clostridium thermocellum gene in one reading frame. Меthods. Measurement of fresh weight (FW), total soluble protein content (TSP) and superoxide dismutase (SOD) activity in leaf tissues. Results. Control (nontransformed) and desС canola plants which were previously propagated by grafting in vitro did not differ significantly on FW, TSP content and SOD activity during growth under +22°С, when temperature was changed to +4°С, аnd after returning to initial temperature conditions (+22°С). Conclusions. Canola plants expressing DesС of cyanobacterium without chloroplast compartmentalization do not change their tolerance to low positive temperature in comparison with nontransformed ones.

Key words: Brassica napus canola, cyanobacterium, desaturases, desC, SOD, low positive temperature.

Author Biography

Л. А. Сахно, Institute of Cell Biology and Genetic Engineering NAS of Ukraine Ukraine, 03680, Kyiv, Zabolotnoho str., 148