Role of salicylate and jasmonate signaling in lipopolysaccharide-induced resistance of Arabidopsis thaliana to the phytopathogenic strain of Pseudomonas aeruginosa IMB 9096

  • J. V. Shilina Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci. Ukraine, Ukraine, 03143, Kyiv, Zabolotnogo str., 148
  • M. I. Guscha Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci. Ukraine, Ukraine, 03143, Kyiv, Zabolotnogo str., 148
  • O. S. Molozhava Educational and Scientific Centre «Institute of Biology» of Taras Shevchenko National University, Ukraine 03022, Kyiv, Glushkov prospect, 2
  • S. V. Litvinov Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci. Ukraine, Ukraine, 03143, Kyiv, Zabolotnogo str., 148
  • A. P. Dmitriev Institute of Cell Biology and Genetic Engineering, Natl. Acad. Sci. Ukraine, Ukraine, 03143, Kyiv, Zabolotnogo str., 148
DOI: https://doi.org/10.7124/visnyk.utgis.15.1.713

Abstract

Aim. The aim of the investigation was to study the effect of lipopolysaccharides (LPS) derived from saprophytic strains of Pseudomonas aeruginosa on the resistance to phytopathogenic strain of P. aeruginosa IMB 9096. The wild-type (Col-0) Arabidopsis thaliana plants, npr1 mutant, which lacks expression of PR-genes, NahG genotype plants, expressing the bacterial gene of NahG salicylate hydrolase, jin1 mutant, insensitive to jasmonic acid, have been used as a model systems in resistance testing. Methods. Common phytopathological methods were used. Results. Lipopolysaccharide from the saprophyte P. aeruginosa IMV 8614 strain increased the resistance of seedlings of all genotypes to infection with phytopathogenic strain P. aeruginosa IMB 9096. The most effective protection had been observed in the mutant jin1. The protective effect was also observed in jin1 after the treatment with LPS derived from the saprophyte strain P. aeruginosa IMV 8615. LPS 8615 increased the sensitivity to infection in the NahG and npr1 transgenic plants, especially in NahG. LPS from the saprophyte P. aeruginosa IMV 8616 increased resistance to P. aeruginosa IMB 9096 infection in all four A. thaliana genotypes. Conclusions. The effect of LPS derived from different strains of saprophytic bacteria can both increase and decrease the sensitivity of plants to infection with bacterial phytopathogens. The effect of LPS depends upon the bacteria strain and the functional state of the salicylate and jasmonate signaling systems in the infected plants.

Keywords: Arabidopsis thaliana, Pseudomonas aeruginosa, lipopolysaccharide, induced resistanse

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