Evaluation of the green pea (Pisum sativum L.) collection for presence of carriers of r and rb mutations

  • A. A. Vasylenko Plant Production Institute nd. a. V. Ya. Yuryev NAAS, 61060, Ukraine, Kharkiv, Moskovskyi pr., 142
  • S. M. Tymchuk Academy of Engineers of Ukraine, 61046, Ukraine, Kharkiv, Dm. Pozharsky str. 2/10
  • V. V. Pozdnyakov Plant Production Institute nd. a. V. Ya. Yuryev NAAS, 61060, Ukraine, Kharkiv, Moskovskyi pr., 142
  • O. Yu. Derebizova Plant Production Institute nd. a. V. Ya. Yuryev NAAS, 61060, Ukraine, Kharkiv, Moskovskyi pr., 142
  • I. M. Bezuglyi Plant Production Institute nd. a. V. Ya. Yuryev NAAS, 61060, Ukraine, Kharkiv, Moskovskyi pr., 142

Abstract

Objective. To evaluate the green pea (Pisum sativum L.) collection in order to identify carriers of r and rb mutations in it. Methods. Pea accessions were grown in the field, and seeds were produced under controlled pollination. The starch granule shape was determined in micrographs taken with a computer digital microscopic camera DMC-300 through a Biolam-15 microscope (object lens × 40). The starch content was determined polarimetrically by the Evers method and the amylase content colorimetrically — by the BO Juliano method. The data were statistically processed using analysis of variance. Results. The effect of r and rb mutations on the starch content and fractional composition in pea seeds was confirmed. In smooth-seed accessions the average starch content was 39,9 %, and the amylose content in starch was 45,9 %, while in r mutation carriers these contents were 31 ,2 % and 67,6 %, respectively, and in rb mutation carriers — 26,9 % and 28,2 %, respectively. An accession with marrowfat seeds, roundish starch granules and a low content of amylose in starch was identified. It was established that this accession was a carrier of a recessive mutation that is not allelic to r mutation and coincides with rb mutation by the effect type. Conclusions. A new genetic source of starchmodifying monogenic rb mutation of pea was identified. It was involved hybridization with sources of r mutation to expand the genetic basis of pea cultivar breeding.

Keywords: peas, Pisum sativum, genetic diversity, r, rb, seed phenotype, starch grain shape, starch content, amylose content.

References

Merezhko A.F. Printsipy poiska, sozdaniia donorov tsennykh priznakov v selektsii rasteniy. Identifitsirovannyy genofond rasteniy i selektsiia. St.Petersburg, 2005. P. 189–205.

Andreev N.R. Osnovy proizvodstva nativnykh krakhmalov (nauchnye aspekty). Moskva: Pishchepromizdat, 2001. 289 p.

Carbohydrates in grain legume seeds: Improving nutritional quality and agronomic characteristics. C.L. Hedley Ed. Wallingford, UK: CAB Int. Publ., 2001. 322 p.

Casey R. Domoney C., Smith A.M. Biochemistry and molecular biology of seed products. Peas: genetics, molecular biology and biotechnology; R. Casey, D.R. Davies Eds. Cambridge: CAB Int., 1993. P. 121–164.

Jansman A.J.M. Bioavailability of proteins in legume seeds. Grain Legumes. 1996. No 11. P. 18-19.

Starch chemistry and technology, 3rd ed. / J. BeMiller, R. Whistler Eds. Acad. Press, 2009. 900 p.

Hedley C.L., Bogracheva T.Ya., Lloyd J.R., Wang T.L. Manipulation of starch composition and quality in pea seeds. Agri-food quality: an intrerdisciplinary approach. G.R. Fenwick, C.L. Hedley, R.C. Richards, S. Khorkar Eds. Cambrige: Royal Sos. Chem., 1996. P. 138–148.

Boyer C.D., Hannah L.C. Kernel mutants of corn. Specialty Corns; A.R. Hallauer Ed. Boca Raton London New-York Washington, D.C.: CRC Press, 2001. P. 10-40.

Gritton E.T. Pea breeding. Breeding vegetable crops: M.J.Basset Ed. Westport, CT: AVI Publ. Co., 1986. P. 283-319

Genetic improvement of vegetable crops. G.Kaloo, B.O.Berght eds. Oxford: Pergamon Press, 1993. 833 p.

Vasylenko A.O., Derebizova O.Yu., Tymchuk S.M., Pozdniakov V.V., Tymchuk V.M. Vmist sukhoi rechovyny ta tsukriv u tekhnichno styhlomu zerni riznykh sortiv ovochevoho gorokhu. Tavriyskyy naukovyy visnyk. 2011. No 76. P. 26-33.

Wang T.L., Hedley C.L. Genetic and developmental analysis of the seed. Peas: genetics, molecular biology and biotechnology; R. Casey, D.R. Davies Eds. Cambridge: CAB Int., 1993. P. 83–120.

Martin C., Smith A. Starch biosynthesis. The Plant Cell. 1995. Vol. 7. P. 971-985. doi: 10.1105/tpc.7.7.971

Hylton C., Smith A.M. The rb mutation of peas causes structural and regulatory changes in ADP glucose pyrophosphorylase from developing embryos. Plant Physiol. 1992. Vol. 99(4). P. 1626-1634.

Wang T.L. Bogracheva T.Y., Hedley C.L. Starch: as simple as A, B, C? J. Exp. Bot. 1998. Vol. 49. P. 481–502.

Lloyd J.R., Wang T.L., Hedley C.L. An analysis of seed development in Pisum sativum. XIX. Effect of mutant alleles at the r and rb loci on starch grain size and on the content and composition of starch in developing pea seeds. J. Exp. Bot. 1996. Vol. 47. P. 171 -180.

Dospekhov B.A. Metodika polevogo opyta. Moskva: Agropromizdat, 1985. 351 p.

Metody biokhimicheskogo issledovaniia rasteniy / Ed. By A.I. Ermakov. Leningrad: Agropromizdat, 1987. 430 p.

Juliano B.O. A simplified assay for milled-rice amylase. Cereal Sci. Today. 1971. Vol. 16. P. 334–340.

Lakin G. F. Biometriia. Moskva: Vysshaia shkola, 1973. 343 p.

Weeden N.F., Wolko B. Linkage map for the garden pea (Pisum sativum L.). Genetic maps. Locus maps of complex genomes, 5th ed.; S.J.O’Brien Ed. New-York: Cold Spring Harbor Lab. Press, 1990. P. 6.106-6.112.