Activity of ascorbate and guaiacol peroxidases in Arabidopsis thaliana Cat2 knockout mutants under salt stress

  • N. O. Didenko Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Chernivtsi, Kotsubynski str., 2
  • I. M. Buzduga Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Chernivtsi, Kotsubynski str., 2
  • R. A. Volkov Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Chernivtsi, Kotsubynski str., 2
  • I. I. Panchuk Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Chernivtsi, Kotsubynski str., 2

Abstract

Aim. Plant stress proteins are usually encoded by multigene families. However, the specific roles of the individual isoenzymes are still poorly understood. To elucidate how the loss of some catalase isoforms affects the function of other enzymes that destroy hydrogen peroxide, namely ascorbate and guaiacol peroxidases (APX and POD), the response to salt stress in Arabidopsis thaliana wild type (WT) and Cat2 knockout mutant was compared. Methods. The plants were subjected to various regimes of sodium chloride treatment and the activity of APX and POD was measured. Results. It was found that the activity of POD in the leaves increased after 8 hours of treatment in WT plants and after 4 hours of treatment in cat2 knockout line. Activity of APX in WT plants decreased after 8 hours treatment with 200 mM NaCl, but remained unchanged in the cat2 line. Conclusions. The decreased catalase activity in leaves of cat2 knockout mutant of Arabidopsis leads to a readjustment of antioxidant systems (in particular – changes in activity of peroxidases) and accelerates the onset of a stress response to salt stress.

Keywords: Arabidopsis thaliana, multigene families, knockout mutants, ascorbate and guaiacol peroxidases, catalase, isoenzymes, sodium chloride.

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