Cell population genetics: emergence, main results and concepts (to the 50th anniversary of the foundation)

  • V. A. Kunakh Institute of Molecular Biology and Genetics of the NAS of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150


The article briefly reviews the emergence and development in Ukraine of a new scientific field — cell population genetics, which formed the theoretical basis of modern cell technologies. These include, in particular, plant biotechnologies for the improvement, preservation, and accelerated reproduction of unique genotypes in vitro; development of new genotypes (organisms) by the methods of cell and genetic engineering and cellular selection; obtaining biologically active compounds, including recombinant, from biomass of cultured cells and tissues for the needs of medicine, cosmetics and food industry; as well as the methods of cell therapy, including technologies based on the use of stem cells, etc. Cultured cells are widely used as model objects and biological systems for studying the most relevant problems of modern biology: the features of the course, signaling pathways, and mechanisms of cell proliferation, including carcinogenesis and tumor proliferation; dedifferentiation of cells, including their reversion to a pluripotent state; totipotency, pluripotency and omnipotency; regeneration of tissues, separate organs, and whole organisms, etc. The article reviews scientific prerequisites for the development of the new scientific field and presents the main concepts of cell population genetics, which have been mainly developed in the Department of cell population genetics of the Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine. It also presents an analysis of the features of development of the newest research areas of genetics of somatic cells of intact plants and cells in vitro, cell population genetics, genetic foundations of cell selection, cell biology, and biotechnology during the second half of the past and the beginning of this century.
Keywords: history of science, cell population genetics, plant tissue and cell culture, cell selection, plant biotechnology.


Butenko R. G. First All-Union conference of culture of isolated plant organs, tissues and cells. Moscow, 22-26 January 1968. Physiology of Plants (Moscow). 1968. V. 15, N. 5. P. 941-943. (In Russian).

Kultura izolirovannyh organov, tkanej i kletok rastenij. Proceedings of the First All-Union Conference. Moscow, January 22-26, 1968. R.G. Butenko (Ed.). Moskva, Nauka, 1970. 342 р. (In Russian).

Kunakh V.A. Osnovni napryamy moyikh naukovykh doslidzhenʹ za 50 rokiv (1966 – 2016 rr.). Kunakh Viktor Anatoliyovych: Biobibliohrafichnyy pokazhchyk naukovykh pratsʹ za 1966 – 2016 roky /uklad. L.P. Mozhylevska, M.Z. Mosula, I.O. Andryeev. N.M. Drobyk (Ed.). Ternopilʹ: Pidruchnyky i posibnyky, 2017. P. 25-95. (In Ukrainian).

Gautheret R.J. La culture des tissues vegetaux. Techniques et realistions. Paris, 1959. 868 p.

Gautheret R.J. Histogenesis in plant tissue cultures. J. Nat. Cancer Inst. 1957. Vol. 19. P. 555-564.

Buvat R. Recherches sur la dedifferenciation des cellules vegetales. Ann. Sc. Nat. Bot. Biol. veg. 1944. No. 5. P. 1-130.

Buvat R. Recherches sur la dedifferenciation des cellules vegetales Ann. Sc. Nat. Bot. Biol. veg. 1945. No. 6. P. 1-119.

Bartos J. Dëleni bunek v kulturach casti korene mrkve (Daucus carota). Čescosl. biol. 1954. No. 3. P.206-211.

Tulecke W.R. The pollen of Ginkgo biloba. In vitro culture and tissue formation. Am. J. Bot. 1957. V. 44, No. 5. P. 602-608.

Melchers G., Bergman L. Untersuchungen an Kulturen von haploiden Geweben von Antirrhinum majus. Ber. Dtsch. Bot. Ges. 1959. V. 71, N. 4. P. 459-473.

Straus J. Maize endosperm tissue grown in vitro. II. Morphology and Cytology. Am. J. Bot. 1954. V. 41. P. 833-839.

Partаnen C.R. Quantitative chromosomal changes and differentiation in plants. Developmental cytology. New York: Ronald Press. 1959. P. 21-45.

Partаnen C.R. Plant tissue culture in relation to developmental cytology. Intern. Rev. Cytol. 1963. Vol. 15. P 215-243.

Mitra J., Mapes M.O., Steward F.C. Growth and organised development of cultured cells. IV. The behaviour of the nucleus. Am. J. Bot. 1960.Vol. 47, No. 5. P. 357-368.

Torrey J.G. Differential mitotic response of diploid and polyploid nuclei to auxin and kinetin treatment. Science. 1958. Vol. 128. P. 1148.

Torrey J. G. Experimental modification of development in the root. Cell, organism and milieu. N. Y. Roland Press. 1959. P. 189-222.

Torrey J. G. Kinetin as trigger for mitosis in nature endomitotic plant cells. Exptl. Cell Res. 1961. V.23. P. 281-299.

Kallak H.I., Jarvekulg L.J. On the morphological and cytological variety of Pea callus. Cytol. Genet. 1968. Vol. 2, No. 5. P. 408-414.

Kallak H.I., Jarvekulg L J. Cytogenetic studies on pea callus. Kultura izolirovannyh organov, tkanej i kletok rastenij. R.G. Butenko (Ed.). Moskva, Nauka, 1970, P. 140-143 (in Russian).

Mitra J., Steward F.C. Growth induction in cultures of Haplopappus gracillis. II. The behavior of the nucleus. Am. J. Bot. 1961. Vol. 48, No. 5. P. 358-368.

Venketeswaran S. Tissue culture studies of Vicia faba. II. Cytology. Caryologia. 1963. Vol. 16, No. 1. P. 91-100.

Cooper L.S., Cooper D.C., Hilderbrandt A.S., Ricker A.J. Chromosome numbers in single cell clones of tobacco tissue. Am. J. Bot. 1964. Vol. 51, No.3. P. 284-290.

Kallak H.I. Cell division and chromosome numbers in the tissue culture of Nicotiana tabacum. Biol. Plant. 1968. Vol. 10, No. 3. P. 199-204.

Patau K., Das N.K. The relation of DNA synthesis and mitosis in tobacco pith tissue cultured in vitro. Chromosoma. 1961. Vol. 11. P. 553-572.

Fox J. Growth factor requirements and chromosome number in tobacco tissue culture. Physiol. Plant. 1965. Vol. 16, No.4. P. 793-803.

Melchers G. Einige genetische Gesinchtspunkte su sogenanuten Gewebekulturen. Ber. Dtsch. Bot. Ges. 1965. B. 78, No. 11. P. 21-29.

Murashige T., Nakano R. Morphogenetic behavior of tobacco tissue culture and implication of plant senescence. Am. J. Bot. 1962. V. 52, No. 8. P. 819-827.

Shamina Z. B., Butenko R. G., Tarasov V. A. Cytological investigation of s tissue-culture of the tobacco plant. Genetika (Moscow). 1966. No. 1. P. 70-76 (in Russian).

Sacristan M.D. Auxin-Autotrophoe und Chromosomen Zahl Untersuchungen an alten, spontan habituierten und crowngall Kallusskulturen von Nicotiana tabacum aus dem Laboratorium Gautherets. Molec. Gen. Genet. 1967. Vol. 99, No. 4. P. 311-321.

Shimada T., Tabata M. Chromosome numbers in cultured pith tissue of tobacco. Japan J. Genet. 1967. Vol. 42, No. 4. P. 13-18.

Vasil V., Hildebrandt A.C. Further studies on the growth and differentiation of single, isolated cells of tobacco in vitro. Planta. 1967. Vol. 75, No. 2. P. 139-151.

Kallak H. Cell division and chromosome numbers in the tissue culture of Nicotiana tabacum. Acta. Biol. Acad Sci. Hunh. 1968. Vol. 22, No. 1. P. 67-73.

Asuwa N. Progressive change in organ-forming capacity of tobacco callus during single subculture period. Japan J. Genet. 1972. Vol. 47, No. 1. P. 53-60.

Jeoman M.M., Evans P.K., Naik G.G. Changes in mitotic activity during carly callus development. Nature. 1966. Vol. 209, No. 5028. P. 1115-1116.

Murashige T., Nakano R. Chromosome complement as a determinant of the morphogenic potential of tobacco cells. Am. J. Bot. 1967. Vol. 54, No. 8. P. 963-970.

Kallak H.I., Jarvekulg L. J. Cytogenetic characteristic of some strains of callus pea. Genetic of grain legumes. Orel. 1972. P. 7-16 (in Russian).

Zossimovich V.P., Levenko B.A., Yurkova G.N., Legeida V.S. Isolation of strains of Crepis capillaris of different ploidy in tissue culture. Rep. Acad. Sci. USSR. Ser. Biology. 1972. Vol. 203, No. 4-6. P. 1188-1189 (in Russian).

Blakely L., Steward F. Growth and organized development of cultured cells. VII. Cellular variation. Am. J. Bot. 1964.Vol. 51, No. 7. P. 809-817.

Jamada T., Shoji T., Sinoto J. Cytological studies on cultured cells. II. Formation of calli and general behavior of their cells in the tissue culture of Tradescantia paludosa. Bot. Mag. Tokyo. 1964. Vol. 77, No. 918. P. 436-446.

Shamina Z.B., Frolova L.V. Cytogenetic studies on tissue culture of Haplopappus during prolonged cultivation. Kultura izolirovannyh organov, tkanej i kletok rastenij. R.G. Butenko (Ed.). Moskva: Nauka, 1970. P. 149-154 (in Russian).

Kunakh V.A. Cytogenetic behavior of tissue culture of Haplopappus. Kultura izolirovannyh organov, tkanej i kletok rastenij. R.G. Butenko (Ed.). Moskva: Nauka, 1970. P. 155-158 (in Russian).

Sidorenko P.G., Kunakh V.A. Character of caryotype variability in cell population of tissue culture of Haplopappus gracilis with long-term passaging. Cytol. Genet. (Tsitologiya i genetika). 1970. Vol.4, No. 3. P. 235-241 (in Russian).

Kunakh V.A. Cytogenetic differences in strains of tissue cultures of leaf and stem origin in Haplopappus gracilis (Nutt.) Gray. Cytol. Genet. (Tsitologiya i genetika). 1971. V.5, No. 3. P. 241-249. (in Russian).

Kunakh V.A., Mozhilevskaja L.P. Wlijanije ishodnogo materiala i pitatelnoj sredy na hromosomnuju ismenchivost kletok Haplopappus gracilis v culture in vitro. Voprosy molekularnoy biologii i genetiki. S.M. Gershenson (Ed). Kiev: Nauk. Dumka. 1972. P. 17-18 (in Russian).

Sidorenko P. G., Kunakh V. A. Production of culture of isolated tissues of Haplopappus gracilis and Crepis capillaris and their cytogenetic characteristic. Cytol. Genet. (Tsitologiya i genetika). 1972. Vol. 6, No. 6. P. 483-486. (in Russian).

Gupta S. Tissue culture of Nigella sativa. I. The behaviour of nucleas. Experientia. 1972. Vol. 28, No. 4. P. 441-445.

Ravkin A. S., Popov Ya. Cytogenetic activity, genomic and chromosomal changes in cells apple tree tissue culture. Cytol. Genet. (Tsitologiya i genetika). 1973. Vol. 7, No. 1. P. 33-36. (in Russian).

Zagorska N.A., Shamina Z.B., Butenko R.G. Investigation of tobacco plants obtained by regeneration from a tissue culture. Genetika (Moscow). 1971. Vol. 7, No. 3. P. 23-63 (in Russian).

Staudt G., Borner H. C., Becker H. Untersuchungen über die Kallusbilldung von di- und tetraploiden Reben in vitro. Vitis. 1972. Vol. 1. P. 1-9.

Srivastava P. S. In vitro induction of triploid roots and shoots from nature endosperm of Jatropa panduraefolia. Z. Pflansenphysiol. 1971. Vol. 66, No. 1. P. 93-96.

Torok D., Roderick T. N. Association between growth rate, mitotic frequency and chromosome number in a plant tissue culture. Cancer Res. 1962. Vol. 22, No. 2. P. 174-181.

Bennici A., Buiatti N., D’Amato P., Paglial M. Nuclear behavior in Haplopappus gracilis callus grown in vitro on different culture media. Collq. Int. CNRS. 1968. Vol. 193. P. 245-250.

Binding H., Binding K., Straub J. Selektionen Gewebekulturen mit haploiden Zellen. Naturwiss. 1970. Vol. 57, No. 3. P. 138-139.

Zenkteler M. A., Gurowska J. Cytological studies on the regenerating nature female gametophyte of Taxus baccata T. and nature endosperm of Tilia platyphylles Scop. in vitro culture. Acta Soc. Botan. Polon. 1970. Vol. 39, No. 1. P. 161-175.

Sacristan M. D. Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris (L.) Wallr. Chromosoma. 1971. Vol. 33. P. 275-283.

Levenko B.A., Kunakh V.A., Yurkova G.N. Tsitogenetitscheskoje izutschenije kallusnoj tkani haploidhoho proishozhdenija. Eksperimentalnaja poliploidija u kulturnyh rastenij. V.P. Zossimovich (Ed.). Kiev: Naukova dumka. 1974. P. 173-180 (in Russian).

Mar’yakhina I.A., Butenko R.G. Somaticheskaya reduktsiya v culture tkani kapusty. Tsitologiya i genetika (Cytol. Genet.) 1974. Vol. 8, No. 3. P. 267-269 (in Russian).

Kunakh V.A. Poliploidija v kulture kletok in vitro i jejo wozmozhnyje pritschiny. Eksperimentalnaja poliploidija u kulturnyh rastenij. V.P. Zossimovich (Ed.). Kiev: Naukova dumka, 1974. P. 39-57 (in Russian).

Kunakh V.A. Cytogenetic studies on cell populations in culture of isolated plant tissues: Ph. D. Thesis (03.00. 15–Genetics). Kiev, 1975. 16 P. (in Russian).

Zossimovich V.P., Kunakh V.A. Frequency, type and origin of chromosome aberrations in plant tissue cultures. Genetika (Moscow). 1975. Vol. 11, No. 6. P.37-46 (in Russian).

Kunakh V. A. Features of structural mutagenesis in cultured plant cells populations. Uspekhi sovrem. genetiki. N. P. Dubinin (Ed.). Moscow: Nauka. 1984. Vol. 12. P. 30-62.

Eriksson T. Physiological and cytological studies on cell cultures of Haplopappus gracilis. Acta univ. Upsal. Abstr. Diss. Sci. 1967. Vol. 93. P. 11.

Kovalyeva T.A., Shamina Z. B., Butenko R. G. Cytological studies of the tissue culture of Rauwolfia serpentina Benth. Genetika (Moscow). 1968. Vol. 4, No. 5. P. 5-13 (in Russian).

Kovalyeva T.A. Cytomorphological studies on tissue culture of Rauwolfia serpentina Benth. Kultura izolirovannyh organov, tkanej i kletok rastenij. R.G. Butenko (Ed.). Moskva: Nauka, 1970. P. 136-139. (in Russian).

Sacristan M.D., Wendt-Gallitelli M.P. Tumorous cultures of Crepis capillaris. Chromosoma. 1973. Vol.43, No. 3. P. 279-288.

Norstog K., Wall W., Howland C. Cytological characteristics of ten-year-old ryegrass endosperm tissue culture. Bot. Ges. 1969. Vol. 130, No. 2. P 83-86.

Kunakh V.A. Biotechnology of medicinal plants. Genetic, physiological and biochemical basis. Kyiv: Logos, 2005. 724 p. (in Ukrainian).

Libbenga K.E., Torrey J.W. Hormone-induced endoreduplication prior to mitosis in cultured pea root cortex cell. Am. J. Bot. 1973. Vol. 60, No. 4. P. 293-299.

Phillips R., Torrey J. DNA synthesis, cell division and specific cytodifferentiation in cultured pea root cortical explants. Developm. Biol. 1973. Vol. 31, No. 2. P. 336-347.

Reinert J., Torrey J. Űber die Kultur von Geweben aus Haplopappus gracilis. Naturwissenschaf. 1961. Vol. 48, No. 5. P. 132-133.

Venketeswaran S., Spiess E. B. Tissue culture on Vicia faba. III. Effect of growth factors on chromosome morphology. Cytologia. 1963. Vol. 28, No. 2. P. 201-212.

Torrey J.G., Reinert J., Merkel N. Mitosis in suspension cultures of higher plant cells in a synthetic medium. Am. J. Bot. 1962. Vol. 49, No. 4. P. 420-425.

Kallak H., Yarvekylg L. On the cytogenetic effects of 2,4-D on pea callus in culture. Acta Biol. Acta Sci. Hung. 1971. Vol. 22, No. 1. P. 67-75.

Bennici A., Buiatti N., D'Amato F., Pagliai M. Nuclear behavior in Haplopappus gracilis callus grown in vitro on different culture media. Cells Int. CNRS. 1971. Vol. 193. P. 245-250.

Demoise C., Partanen C. Effect of subculturing and physical condition of medium on the nuclear of a plant tissue culture. Am. J. Bot. 1969. Vol. 56, No. 2. P. 147-152.

Wright K., Northcote D. H. Differences in ploidy and degree of intercellular contact in differentiating and non differentiating sycamore calluses. J. Cell Sci. 1973. Vol. 12, No. 1. P. 37-53.

Strogonov B.P., Butenko R.G., Shamina Z.B., Kulieva F.B. Cytogenetic action of NaCl on the culture of tissue of Crepis capillaris. Rep. Acad. Sci. USSR, Ser. Biology. 1973. Vol. 209, No. 1-3. P. 243-245 (in Russian).

Hsu T.C., Kellog D.S. Jr. Mammalian chromosomes in vitro. XII. Experimental evolution of cell populations. J. Nat. Cancer Inst. 1960. Vol. 24, No. 5. P. 1067-1093.

Hsu T.C. Chromosomal evolution in cell populations. Internat. Rev. Cytology. 1962. Vol. 12. P. 69-161.

Hughes D.T. Cytogenetical polymorphism and evolution in mammalian somatic cell populations in vivo and in vitro. Nature. 1968. Vol. 217. P. 518-523. Doi: 10.1038/217518a0.

Olenov Yu.M. Kletochnaya nasledstvennost, differentsirovka kletok i kantserogenez kak problemy evolyutsionnoy genetiki. L.: Nauka. 1967. 310 p. (in Russian).

Vakhtin Yu.B. Geneticheskaya teoriya kletochnykh populyatsiy. L.: Nauka. 1980. 168 p. (in Russian).

Vakhtin Yu.B, Pinchuk V.G., Shvemberg I.N., Butenko Z.A. Klonal'no-selektsionnaya kontseptsiya opukholevogo rosta. Kiev: Naukova dumka. 1987. 216 p. (in Russian).

Dobzhansky Th. Mendelian populations and their evolution. Genetics in 20th century. U.C. Dunn (ed.) New York: Acad. Press. 1952. P. 573-589.

Gavrilov V. I. Perevivayemyye kletki v virusologii. Moskva: Meditsina. 1964. 264 p. (in Russian).

Kunakh V.A. Plastichnost' genoma somaticheskikh kletok i adaptivnost' rasteniy. Molekulyarnaya i prikladnaya genetika. Minsk. 2011. Vol. 12. P. 7-14. (in Russian).

Kunakh V.A. Ontogeneticheskaya plastichnost' genoma kak osnova adaptivnosti rasteniy. Zhebrakovskiye chteniya III. Inst. Genetiki i Tsitologii NAN Belarusi. Otv. red. A.V. Kil'chevskiy. Minsk. 2011. 56 p. (in Russian).

Kordium V.A. Nasha «shagrenevaya kozha» - eto nasha problema. Nam yeye i reshat'. Kiev: Logos. 2006. 264 p. (in Russian).

Solov'yan V.T., Spiridonova K.V., Kunakh V.A Genome rearrangements in cultured Rauwolfia serpentina cells: Diverse pattern of genome variation. Genetika (Moscow). 1994. Vol. 30, No. 2. P. 250-254. (in Russian).

Solov’yan V.T., Spiridonova K.V., Kunakh V.A. Genome rearrangements in cultured Rauwolfia serpentina cells. II. Relation to interspecific variation. Genetika (Moscow).. 1994. Vol. 30, No. 3. P. 399-403. (in Russian).

Solov’yan V.T., Spiridonova K.V., Kunakh V.A. Special features of genomic variation of cell culture of Rauwolfia serpentina. Cytol. Genet. 1994. Vol. 28, No. 5. P. 21-25.

Kunakh V.A. Evolyutsiya genomu roslyn v kylturi klityn in vitro. Osoblyvosti, prychyny, mekhanizmy ta naslidky. Henetika i selektsiya v Ukrayini na zlami tysyacholit. V.V. Morgun et al. (Eds.). K.: Logos. 2001. P. 53-67.

Kunakh V.A. Mechanisms and some regularities to somaclonal variability of plants. Visn. Ukr. tov. genet.sel. 2003. No. 1. P. 101-106. (in Ukrainian).

Andreev I.O., Spiridonova K.V., Kunakh V.A Plant genome rearrangements in cell in vitro. Biopol. Cell. 2004. Vol. 20. No. 1-2. P. 42-49.

Andreev I.O., Spiridonova K.V., Solov’yan V.T., Kunakh V.A. Variability of ribosomal RNA genes in Rauwolfia species: parallelism between tissue culture-induced rearrangements and interspecies polymorphism. Cell Biol. Internat. 2005. Vol. 29. P. 21-27.

Kunakh V.A., Andreev I.O., Spiridonova K.V. 18S-25S and 5S рРНК interspecies polymorphism and variability in tissue culture of Rauwolfia Benth. and Gentiana L. Physiol. Biochem. Cultiv. Plants. 2009. Vol. 38, No. 2. P. 110-123. (in Ukrainian).

Andreev I.O., Spiridonova K.V., Maidanyuk D.M., Kunakh V.A. Genetic effects of tissue culture on maize. Physiol. Biochem. Cultiv. Plants. 2009. Vol. 41, No. 6. P. 487-495. (in Ukrainian).

Severtsov A.N. Glavnyye napravleniya evolyutsionnogo protsesa. Morfobiologicheskaya teoria evolyutsii. M.-L.: Biomedgiz. 1934. 150 p. (in Russian).

Kunakh V.A. Genome variability in plant somatic cell. III. Callus formation in vitro. Biopol. Cell. 1997. Vol. 13, No. 5. P. 362-371. (in Russian).

Kunakh V.A. Variation of the plant genome upon dedifferentiation and callus formation in vitro. Russ. J. Plant Physiol. 1999. Vol. 46, No. 6. P. 919-929.

Twardovska M.O., Drobyk N.M., Mel’nyk V.M., Konvalyuk I.I., Kunakh V.A. Genome variability of some Gentiana L. species in nature and in culture in vitro: RAPD-analysis. Biopol. Cell. 2010. Vol. 26, No. 6. P. 499-507.

Bublyk O. M., Andreev I.O., Spiridonova K.V. Kunakh V.A. The variability of PCR markers based on the genes of disease resistance and the response to abiotic stress in tissue culture. Fakt. Experimental Evolution of Organisms. V.A. Kunakh et al. (Eds). K.: Logos. 2011. Vol. 11. P. 213-218.

Konvalyuk I.I., Mel’nyk V.M., Drobyk N.M., Kravets N.B., Twardovska M.O., Kunakh V.A. RAPD- and ISSR-analysis of genetic variation of Gentiana pneumonanthe L. tissue and organ culture. Visn. Ukr. tov. genet. sel. 2011. Vol. 9, No. 1. P. 22-31.

Konvalyuk I.I., Drobyk N.M., Mel’nyk V.M., Twardovska M.O., Kravetc N.B., Kunakh V.A. RAPD-and ISSR-analysis of the culture of tissues and organs of Gentiana lutea L. Achievements and problems of genetics, breeders and biotechnology. V. A. Kunakh et al. (Eds). Kyiv: Logos. 2012. Vol. 4. P. 523-527. (In Ukrainian).

Kunakh V. A. Genome variation in plant somatic cells and factors regulating this process. Cytol. Genet. 1980. Vol. 14, No. 1. P. 73-81.

Lutova L.A., Ezhova T.A., Dodueva I.E., Osipova M.A. Genetics of plants development. S.G. Inge-Vechtomov (ed.) 2 ed. revised and enlarged. SPb.: Publ. house N-L. 2010. 432 p. (In Russian).

Gubar E.K., Kunakh V.A. Karyotype variability of cultured Crepis cells (Crepis capillaris L. Wallr.). Genetika (Moscow). 1992. V. 28, N. 6. P. 51-61. (In Russian).

Kunakh V.A. Somaclonal variation in Rauwolfia. Biotechnology in Agriculture and Forestry. V. 36. Somaclonal variation in crop improvement. II. J.J. Bajaj (Ed). Berlin, Heidelberg, New York: Springer. 1996. P. 315-332.

Spiridonova K.V., Andreev I.O., Solov’yan V.T., Kunakh V.A. Peculiarities of some gene rearrangement in the cell culture of Rauwolfia serpentine Benth. in vitro. Dopovidi NAN Ukr. 2000. No. 2. P. 165-170. (In Ukrainian).

Spiridonova K.V., Andreev I.O., Solov’yan V.T., Kunakh V.A. Molekulyarno-biolohichni osoblyvosti henomnykh perebudov v kul’tyvovanykh in vitro klitynah rauvol’fiyi zmiyinoyi. Henetyka i seletsiya v Ukrayini na mezhi tysyacholit’. V.V. Morgun et al. (Eds). 2001. K.: Logos. Vol. 1. P. 422-427.

Twardovska M.O., Strashnjuk N.M., Mel’nyk V.M., Adonin V.I., Kunakh V.A. Chromosomal variability in tissue culture of rare Gentiana species. Cytol. Genet. 2008. Vol. 42, No. 4. P. 12-17.

Bublyk O.M., Adonin V.I., Kunakh V.A. Cytological variability of cell lines Ungernia victoris when grown on nutrient media of different composition. Cytol. Genet. 2008. Vol. 42, No. 1. P. 29-36.

Bublyk O.M., Andreev I.O., Spiridonova K.V., Kunakh V.A. Variability of Ungernia victoris morphogenic and non-morphogenic tissue culture as results from RAPD-analysis. Visn. Ukr. tov. genet. sel. 2008. Vol. 6, No. 1. P. 44-51. (In Ukrainian).

Kunakh V.A. Mobile genetic elements and plant genome plasticity. Kyiv: Logos. 2013. 298 p. (In Ukrainian).

Kunakh V.A., Melnyk V.M., Drobyk N.M., Andreev I.O., Spiridonova K.V., Twardowska M.O., Konvalyuk I.I., Adonin V.I. Chapter 9. Genetic variation induced by tissue and organ culture in Gentiana species. The Gentianaceae. Volume 2: Biotechnology and Applications. J.J. Rybczynski et al. (Eds). Berlin, Heidelberg: Springer-Verlag. 2015. P. 199-238.

Kunakh V.A., Zossimovich V.P. Effect of kinetin on the frequency and types of chromosome aberration in a tissue culture of Haplopappus gracilis. Genetika (Moscow). 1977. Vol. 13, No. 8. P. 1355-1365. (in Russian).

Kunakh V.A., Sidorenko P. G., Zossimovich V. P. Effect of kinetin on the reproduction of cells of different ploidy. Uspekhi poliploidii. V.P. Zossimovich et al. (Eds.). Kiev: Nauk. Dumka. 1977. P. 203-215. (in Russian).

Brodskyi V.Ya. Formy izmenchivosti v kletochnoy populyatsii takiye zhe, kak i v populyatsii organizmoyv. Ontogenez (Moscow). 1994. Vol. 25, No. 5. P. 29-43. (in Russian).

Mamaeva S.E. Regularities of cell karyotypic evolution in culture. Tsitologia. 1996. Vol. 38, No. 8. P. 787-814. . (in Russian).

Braun B. Genetics of bacteria. Moscow: Nauka. 1968. 446 p. (in Russian).

Kunakh V.A. Genome variability in plant somatic cell. 4. Variability in the process of dedifferentiation and callus formation in vitro. Biopol. Cell. 1998. Vol. 14, No. 4. P. 298-319. (in Russian).

Kunakh V.A. Genome variability in the somatic plant cells. 6. Variability and selection in the course of adaptation to in vitro conditions. Biopol. Cell. 2000. Vol. 16, No. 3. P. 159-185. (in Russian).

Kunakh V.A. Genome variability in somatic plant cells. 7. Variability of population-genetic parameters in the culture in vitro. Biopol. Cell. 2002. Vol. 18, No. 5. P. 377-393. (in Ukrainian).

Kunakh V.A. Biotechnology of plants for improvement of human living conditions. Biotechnol. Acta. 2008. Vol. 1, No. 1. P. 28-39. (in Ukrainian).

Kunakh V.A., Mozhylevskaya L.P. Life expectancy of a person and plant biotechnology. Mol. Applied Genetics. Minsk. 2013. Vol. 14. P. 56-62. (in Russian).

Drobyk N.M., Mel’nyk V.M., Twardowska M.O., Konvalyuk I.I., Kunakh V.A. Chapter 13. Tissue and organ cultures of Gentians as potential sources of xantones and flavonoids. The Gentianaceae. Volume 2: Biotechnology and Applications. J.J. Rybczynski et al. (Eds). . Berlin, Heidelberg: Springer-Verlag. 2015. P. 307-317.

Kunakh V.A. Twenty five years long stable biosynthesis of ajmaline by related hormone-independed Rauwolfia serpentina cell lines. Euromedica –Hannover, 2005. Hannover, 16-17 Juni, 2005. International Congress and Exhibition. Programm, Abstracts. P.22.

Kunakh V.A., Katsan V.A. Biosynthesis of poppy isoquinoline alkaloids in nature and in the in vitro culture. 1. Opium poppy (Papaver somniferum). The Ukr. Biochem. J. 2003. Vol. 75, No. 5. P. 41-54. (in Russian).

Kunakh V.A., Katsan V.A. Biosynthesis of poppy isoquinoline alkaloids in nature and in the in vitro culture. 2. Bracteum poppy (Papaver bracteatum Lindl.). The Ukr. Biochem. J. 2004. Vol. 76, No. 5. P. 29-44. (in Russian).

Miriuta N.Yu., Parnikoza I.Yu., Ammouri Yu., Kunakh V.A. Application of thermodynamic approach to investigation of the in vitro cell population dynamics in Rauwolfia serpentina Benth., a producer of indole alkaloids, tissue culture. Biotechnologiya. (Moscow). 2006. No. 2. P. 78-95. (in Russian).

Miriuta N.Yu., Kunakh V.A. Dinamika klitinnyh systems in vitro. І. Organizatsiya y chasі ta stabil'nist sistemi kul'turi tkanin rauvlofії zmіїnoї na dobovomu rivnі organizatsії. Biotechnol. Acta. 2011. Vol. 4, No. 5. P. 25-38. (in Ukrainian).

Miriuta N.Yu., Kunakh V.A. Dinamika klitinnyh systems in vitro. ІІ. Organizatsiya u chasі ta stabil'nist sistemi kul'turi tkanin rauvlofії zmіїnoї na pasazhnomu rivnі. Biotechnol. Acta. 2011. Vol. 4, No. 6. P. 18-30. (in Ukrainian).

Miriuta N.Yu., Kunakh V.A. Dynamic of cell population systems in vitro. III. Hypothesis of cell differential process self control and it’s phenomenology. Biotechnol. Acta. 2012. Vol. 5, No. 3. P. 40-51. (in Ukrainian).

Wang J., Tian L., Madlung A., Lee H.S., Chen M., Lee J. et al. Stochastic and epigenetic changes of gene expression in Arabidopsis polyploids. Genetics. 2004. Vol. 167. P. 1961-1973.

Gui Q., Wang J., Xu Y., Wang J. Changes in the expression of duplicated genes in allotetraploid species of the genus Brassica according to the SRAP-cDNA method. Mol. Biol. (Moscow). 2009. Vol. 43, No. 1. P. 3-9.

Cullis C.A. Sreda kak generator adaptivnykh izmeneniy i sovremennyye kontseptsii evolyutsionnoy genetiki. Novosibirsk, Inst. Tsitologii i Genetiki SO RAN. 2000. P. 168-176. (in Russian).

Chen Y., Lowenfeld R., Cullis C.A. An environmentally induced adaptive (?) insertion event in flax. Internat. J. Genet. Mol. Biol. 2009. Vol. 1, No. 3. P. 038-047.

Zhuk O.I. Adaptive evolution of water regime and plant drought tolerance. Fakt. eksp.evol. org. V.A. Kunakh et al. (Eds). Kyiv: Logos. 2010. Vol. 8. P.12-16. (in Russian).

Kravets E.A., Berezhnaya V.V., Sakada V.I., Rashidov N.M., Grodzinskij D.M. Mechanisms of radiation mutagenesis and architectonics of the apical root meristem. Fakt. eksp.evol. org. V.A. Kunakh et al. (Eds). Kyiv: Logos. 2011. V. 10. P. 111-115. (in Russian).

Kashin A.S. Gametofitnyi apomiksis kak neustoychivaya sistema semennogo razmnozheniya u tsvetkovykh. Saratov: Nauchnaya kniga. 2006. 310 p. (in Russian).

Kashin A.S. Tsvetova M.I., Demochko Yu.A. Cytogenetic peculiarities of cell genesis in apical meristems under gametophytic apomixis (using autonomous apomicts of the Asteraceae as an example). Cytol. Genet. 2011. Vol. 45, No. 2. P. 28-40.

Maletskiy S.I., Kolodjazhnaya J.C. Genetic variability in cell populations and its effect on reproductive signs in angiosperms. Epigenetics of plants. Novosibirsk. 2005. P. 87-112. (in Russian).

Poznyak S.I., Yudanova S.S. Agamospermic reproduction of seeds in sugar beet (historical aspect). Fakt. eksp.evol. org. V.A. Kunakh et al. (Eds). Kyiv: Logos. 2011. Vol. 10. P. 292-296. (in Russian).

Maletskiy S.I., Hierarchy of units of heredity, variability, inheritance of features and speciation in plants. Epigenetics of plants. Novosibirsk. 2005. P. 7-53. (in Russian).

Golubovskiyi M.D. The age of genetics: the evolution of terms and concepts. SPb.: Borey Art. 2000. 262 p. (in Russian).

Jablonka E., Lamb M.J. The epigenome in evolution: beyond the modern synthesis. Visn. Ukr. tov. genet. sel. 2008. Vol. 6, No. 2. P. 337-355. (in Russian).

Kolotova T.Yu., Stegnii B.T., Kuchma I.Yu., Dubinina N.V., Golovko A.N., Chaikovsky Yu.B., Voljansky Yu.L. Mechanisms and control of reorganizations of the eukaryotic genome. Kharkov: Collegium, 2004. 264 p. (in Russian).

Grant-Downton R.T., Diskinson H.G. Epigenetics and its implications for plant biology. 1. The epigenetic network in plants. Ann. Bot. 2005. Vol. 96. P. 1143-1164.

Grant-Downton R.T., Diskinson H.G. Epigenetics and its implications for plant biology. 2. The “epigenetic epiphany”: epigenetics, evolution and beyond. Ann. Bot. 2006. Vol. 97. P. 11-27.

Liu Y. Historical and modern genetics of plant graft hybridization. Adv. Genet. 2006. Vol. 56. P. 101-129.

Liu Y. Like father like son. EMBO Rep. 2007. Vol. 8, No. 9. P. 798-803.

Cullis C.A. Mechanisms and control of rapid genomic changes in flax. Ann. Bot. 2005. Vol. 95. P. 201-206.

Chandler V., Stam M. Chromatin conversations: mechanisms and implications of paramutation. Nature. Rev. Genet. 2004. Vol. 5. P. 532-544.

Della Vedova C.B., Cone K.C. Paramutation: The chromatin connection. The Plant Cell. 2004. Vol. 16. P. 1358-1364.

Epigenetics of plants. Novosibirsk. Inst. Cytol. Genet. SB RAS. 2005. 373 p. (in Russian).

Lolle S., Victor J., Young J., Pruitt R. Genome-wide non-mendelian inheritance of extra-genomic information in Arabidopsis. Nature. 2005. Vol. 434. P. 505-509.

Jorgensen R.A. Of genes and genomes: chalenges for the twenty-first century. Frontiers plant sci. 2010. Vol. 1. Article 1. doi: 10.3389/fpls.2010.00001.

Kunakh V. A. Something about plasticity of the genome as the basis of plant adaptability. Achievements and problems of genetics, breeding and biotechnology. V. A. Kunakh et al. (Eds.). Kyiv: Logos. 2012. Vol. 4. P. 128-134.

Kunakh V.A. Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consenquences. Biopol. Cell. 2013. Vol. 29, No. 4. P. 295-310.

Kunakh V.A. On the possibility of applying the law of homological series in the hereditary variability of N.I. Vavilov to cellular populations in vitro. Biotechnological methods in the conservation of biodiversity and plant breeding. Articles Internat. Sci. Conf., Minsk, August 18-20. 2014. P. 152-155. (in Russian)/

Maidanyuk D.M., Andreev I.O., Spiridonova K.V., Kunakh V.A. Genetic polymorphism of the maize somaclonal lines derived from P346 line. Biopol. Cell. 2007. Vol. 23, No. 4. P. 324-331.

Maidanyuk D.M., Andreev I.O., Spiridonova K.V., Kunakh V.A. Genomic variability in maize callus cultures of lines P346 and its derivative somaclonal lines. Biopol. Cell. 2007. Vol. 23, No. 5. P. 416-424.

Bublyk O.M., Andreev I.O., Spiridonova K.V., Kunakh V.A. Genetic variability in regenerated plants of Ungernia victoris. Biol. Plant. 2012. Vol. 56, No. 2. P. 395-400.

Spiridonova K.V., Andreev I.O., Zagruchyk O.M., Navrotska D.O., Twardovska M.O., Drobyk N.M., Kunakh V.A. Genetic stability of microptopagated plants of Deschampsia antarctica Desv. during long-term in vitro culture. Plant Physiol. Genet. (Kyiv). 2016. Vol. 48, No. 6. P. 498-507. (in Ukrainian).

Kunakh V.A., Navrotska D.O.,Twardovska M.O., Andreev I.O. Peculiarities of chromosomal variability in cultured tissues of Deschampsia antarctica plants with different chromosome numbers. Visn Ukr. tov. genet. sel. 2016. Vol. 14, No. 1. P. 36-43. (in Ukrainian).

Kunakh V.A. Relationship between ploidy and spontaneous organogenesis in Crepis capillaris and Haplopappus gracilis strains. Tsitol. Genet.(Cytol. Genet.). 1974. Vol. 8, No. 4. P. 303-308. (in Russian).

Kunakh V.A. Tsitogeneticheskiye osobennosti kul'tury izolirovannykh tkaney i regeneriruyemykh rasteniy v svyazi s perspektivoy primeneniya ikh v selektsii. Novyye metody sozdaniya i ispol'zovaniya iskhodnykh materialov dlya selektsii rasteniy. Kiev: Nauk. dumka. 1979. P. 186-193. (in Russian).

Kunakh V. A. Tsitogeneticheskaya izmenchivost' kletochnykh populyatsiy v kul'ture izolirovannykh tkaney rasteniy. Tkanevyye i kletochnyye kul'tury v selektsii rasteniy. M.: Kolos. 1979. P. 38-51. (in Russian).

Kunakh V.A., Alkhimova E.G., Voityuk L.I. Izmenchivost' chisla khromosom v kallusnykh tkanyakh i regenerantakh gorokha. Tsitol. Genet. (Cytol. Genet.). 1984. Vol. 18, No.1. P. 20-25. (in Russian).

Ignatova S.A. Cell technologies in plant growing, genetics and selection of cultivated plants: tasks, opportunities, system development in vitro. Odessa: Astroprint. 2011. 224 p. (in Russian).

Mitrifanova I.V. Somatic embryogenesis and organogenesis as the basis of biotechnology for the production and preservation of perennial garden crops. Kyiv: Agrarian science. 2011. 344 p. (in Russian).

Cherevchenko T.M., Lavrentyeva A.N., Ivannikov R.V. Biotechnology of tropical and subtropical plants in vitro. Кyiv: Nauk. Dumka. 2008. 560 p. (in Russian).

Dubrovna O.V., Chugunkova T.V., Bavol A.V., Ljalko I.I. Biotechnological and cytogenetic bases for the creation of plants resistant to stress. Kyiv: Logos. 2012. 428 p. (in Russian).

Kuchko A.A., Oliynyk T.M. Somaclonal variation in potatoes. Kyiv: Dovira Publishers. 1998, 192 p. (in Ukrainian).