Characteristics of wheat varieties of Poltava State Agrarian Academy breeding with gene markers that determine important agronomical traits
The aim of the work was to determine alleles of Ppd-А1, Ppd-В1, Ppd-D1, Rht8c, Rht-B1, Rht-D1, Pina-D1, Pinb D1, Wx-A1, Wx-B1, Wx-D1 genes of wheat varieties from the Poltava State Agrarian Academy (PSAA). Methods. DNA extraction, allele-specific and SSR-PCR, electrophoresis in agarose and polyacrilamide gels. Results. The vast majority of varieties (9) can be characterized as Ppd-А1b, Ppd-В1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1a, Pina-D1a, Pinb-D1b, Wx-A1a, Wx-B1a, Wx-D1a. Levada, Tsarychanka, Lyutenka and Orzhytsіa varieties were carriers of Ppd-А1b, Ppd-В1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1b, Pina-D1a, Pinb D1b, Wx-A1a, Wx-B1a, Wx-D1a alleles, and the cultivar Sуdor Kovpak — Ppd-А1b, Ppd-В1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1a, Pina-D1a, Pinb-D1а/b, Wx-A1a, Wx-B1a/b, Wx-D1a — was heterogeneous by genes, which are responsible for the quality of the grain. Conclusions. PSAA winter wheat varieties are characterized by a high degree of homogenity in the alleles of genes that determine important economic and agronomical traits. This fact could be explained by the needs of a certain growing zone and traditional breeding approaches of wheat in the PSAA.
Keywords: Аllele-specific markers; winter bread wheat; dwarfing genes; sensitivity to photoperiod; Wx; Pina and Pinb.
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