Content of phenolic compounds in Acorus calamus L. tissue culture and nutrient culture medium under in vitro conditions

  • A. Z. Revutska Educational and Scientific Centre «Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv, Ukraine, 03022, Kyiv, Akademika Glushkova Avenue, 2
  • V. N. Belava Educational and Scientific Centre «Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv, Ukraine, 03022, Kyiv, Akademika Glushkova Avenue, 2
  • A. V. Golubenko Educational and Scientific Centre «Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv, Ukraine, 03022, Kyiv, Akademika Glushkova Avenue, 2
  • N. Yu. Taran Educational and Scientific Centre «Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv, Ukraine, 03022, Kyiv, Akademika Glushkova Avenue, 2


Aim. To find out the biochemical peculiarities of Acorus calamus L of the two genotypes, acquired from different populations, an analysis of phenolic compounds in explant tissues and in nutrient medium in vitro was conducted. Methods. Plants, acquired by microclonal multiplication were studied. To detect general phenol content, Folin–Ciocalteu reagent was used, for flavonoid content - zirconium chloride crystallohydrate nitrate (IV). Xanthone content was identified by Vysochina G.I. and Kukushkina T.A. methods with our own modifications. The extracts were studied using spectrophotometric measurements. Results. Tissues of A. calamus and the nutrient medium contained different amount of phenolic compounds, depending on parent plant origin and in vitro cultivation duration. Conclusions. Since the explants were cultivated in identical conditions, the difference of phenolic compound content both in tissues and nutrient medium indicates genetic variability of A. calamus plants on population level.

Key words. Acorus calamus, culture in vitro, phenols, flavonoids, xanthones.


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