Effect of medium gelatinized component on the effectiveness of androgenesis in vitro Oryza sativa L.

  • I. S. Zambriborshch Plant Breeding & Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road 3
  • O. L. Shestopal Plant Breeding & Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road 3
  • D. V. Shpak Rice Research Institute at Ukrainian Academy of Agrarian Sciences, Ukraine, 75705, Kherson region, Skadovsk district, Antonovka village
  • A. O. Dobrova 1Plant Breeding & Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road 3
  • S. O. Ignatova 1Plant Breeding & Genetics Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odessa, Ovidiopolskaya road 3
DOI: https://doi.org/10.7124/visnyk.utgis.14.2.684

Abstract

Aims. To study the effect of chemically modified starch D–5aM in the culture medium on the efficiency of androgenesis in vitro in anther culture of rice. Methods. Obtaining of rice double haploid lines by anther culture in vitro. The statistical methods. Results The influence different variants of gellatyne source in culture medium on the processes of induction and regeneration in anther culture of rice were studied. The 119 green plants-regenerants were received. Conclusions. The negative effect on the formation of green regenerants using a gel-forming components of the chemically modified starch D–5aM was shown.

Keywords: rice, anther culture in vitro, callus, regeneration, chemically modified starch.

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