Frequency and spectrum of mutations in the M2-M3 of common winter wheat, induced chiral stereoisomers of nitrosoalkylurea

  • В. В. Моргун Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • О. М. Катеринчук Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • Т. В. Чугункова Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska str., 31/17

Abstract

Aim. In order to expand the class of mutagens that would allow to obtain new mutant forms of crops, we investigated the genetic activity of chiral nitrosoalkylureas on winter wheat for the first time. Methods. We used standard methods of processing seed mutagens, field and laboratory methods for the analysis of plants in the generation of M1-M3, methods of statistical analysis. Results. During the study of mutagens, 28 types of mutations was identified. The greatest number of mutations in both varieties was induced by the action of S(+) stereoisomer at a concentration of 0.05%. The frequency of visible mutations in variety Federer ranged from 3,2 % to 9,0%, in a variety of Kyrene – 5,0 % – 12,6 %. Conclusions. For the first time on winter wheat, the genetic activity of new chiral mutagens was investigated. It is established that stereoisomers R(-­) NMsBU and S(+) NMsBU induce significant number of genetic changes in the wheat plants. The frequency and spectrum of mutations caused by them is comparable with the famous supermutagens that allow recommending new chiral mutagens for use in mutation breeding of wheat.

Keywords: chiral nitrosoalkylureas, mutation frequency, spectrum of mutations, common winter wheat.