Analysis of the G742A polymorphism of BHMT gene in patients with psoriasis
Aim. To analyse the single nucleotide polymorphism G742A in the gene of betaine homocysteine methyltransferase S (BHMT) in patients with psoriasis. Methods. Genealogical information is obtained for 72 probands and 60 persons of the control group. Molecular genetic analysis was performed by PCR-RFLP method. Statistical testing of hypothesis of association between the studied genotypes and disease occurrence, and assessment of the equality of distribution series was conducted using χ2 test at significance levels of 0.05, 0.01 and 0.001. Results. Genotyping showed that the allele and genotype frequencies in the control group were: pG = 0,700, qA = 0,30, GG – 45,0 %, GA – 50,0 %, AA – 5,0 %, while in patients with psoriasis – pG = 0,743, qA = 0,257, GG – 50,0 %, GA – 48,6 %, AA – 1,4 %. The actual distribution of genotypes was statistically different from the theoretically expected distribution of BHMT G742A polymorphism in general group of patients. The frequency distribution of genotypes showed a statistically significant difference between the group of patients with psoriasis and control group (p = 0.007). The frequency of the AA genotype in the control group was higher than in the group of patients with psoriasis without arthropathy (5.0 % vs. 2.2 %, p = 0.029). For G742A polymorphism in patients with age of onset of the disease up to 25 years, the actual distribution of genotypes was significantly different from the theoretically expected at a balance (p <0,01). Conclusion. The results of the BHMT G742A polymorphism study in patients with psoriasis, including a higher frequency of genotypes homozygous for allele А among patients, demonstrate the possibility of its further consideration in the planning of preventive and therapeutic measures.
Keywords: psoriasis, one carbon metabolism, folic acid, homocysteine, methionine, betaine homocysteine methyltransferase S.
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