Screening of winter triticale genotypes for resistance to water deficit in in vitro culture of shoot apical meristems

  • С. В. Пикало V.M. Remeslo Myronivka Institute of Wheat, NAAS of Ukraine Ukraine, 08853, Kyiv region, Myronivka district, Tsentralne vil.
  • М. А. Зінченко Institute of Plant Physiology and Genetics, NAS of Ukraine Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • С. І. Волощук V.M. Remeslo Myronivka Institute of Wheat, NAAS of Ukraine Ukraine, 08853, Kyiv region, Myronivka district, Tsentralne vil.
  • О. В. Дубровна Institute of Plant Physiology and Genetics, NAS of Ukraine Ukraine, 03022, Kyiv, Vasylkivska str., 31/17

Abstract

Aim. To conduct in vitro screening of different genotypes of winter triticale for resistance to water deficit in culture of shoot apical meristems. Methods. By direct recruitment selection the screening of triticale genotypes for resistance to water deficit was conducted using low molecular mannitol concentration 0.2-0.8 M. Results. Concentration of manitol 0.6 M was found to allow differentiating triticale genotypes by the resistance to water deficit. Calluses of 38/1296 line are characterized by relatively high morphogenic potential and biomass increment in the presence of 0.8 M mannitol concentration, which for other genotypes prooved fatal. Conclusions. 38/1296 line can be used as a valuable material for further breeding of winter triticale. Culture of shoot apical meristems can be used as a test-system for triticale genotypes screening for resistance to water deficit.

Key words: Triticale, apical meristems, osmotic stress, resistance.