Increase in frequency of wheat callus cultures regeneration for Agrobacterium-mediated transformation

  • О. М. Гончарук Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska Str., 31/17
  • А. В. Бавол Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska Str., 31/17
  • О. В. Дубровна Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska Str., 31/17

Abstract

Aim is to study the impact of immature embryos age and synthetic dicamba auxin on callus morphogenetic potential in two wheat varieties, Podolyanka and Zymoyarka, during Agrobacterium-mediated transformation. Methods. Optimization of conditions for shoot regeneration during Agrobacterium-mediated transformation. Results. Age of immature embryos was found to significantly influence the incidence of callus formation and cultivar-specificity of this indicator. Replacement of 2,4-D on dicamba in the medium for callus formation and IAA on dicamba in the medium for regeneration has increased frequency of shoots induction 2-3 times during genetic transformation. The largest number of plant-regenerants in a variety Podolyanka was obtained on medium with dicamba at concentration 3 mg/L, while in the variety Zymoyarka – at 2 mg/l. Conclusions. Nutrient medium using synthetic auxin dicamba was optimized, thus allowing to increase the incidence of shoots regeneration during Agrobacterium-mediated transformation of wheat.

Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, immature embryos, dicamba, regeneration.