Evaluation of preimplantation genetic screening after IVF with ICSI in couples over 35 years old

  • М. И. Кононенко Human Reproductive Problems Clinic Ukraine, 03037, Kyiv, Chervonozoryany Prospekt, 17
  • С. В. Денисенко Human Reproductive Problems Clinic Ukraine, 03037, Kyiv, Chervonozoryany Prospekt, 17
  • Н. Г. Горовенко P.L.Shupik National medical academy of post-graduate education 9 Ukraine, 04112, Kyiv, Dorohozhytska St., 9

Abstract

Aim. Aim is to perform the retrospective analysis of the results of preimplantation screening in embryos at the 3rd day of development in vitro obtained in programs of IVF with ICSI in couples of older age groups. Methods. Clinical examination, spermiogram, cytogenetic analysis, morphological evaluation of embrio, fluorescent in situ hybridization (FISH). Results. Evaluation of the efficiency of the method to identify genomic mutations in the embryo prior to implantation has been performed. Molecular cytogenetic analysis of all cells of embryos classified as aneuploid after preimplantation diagnosis, showed the prevalence of mosaic embryos (63.6 %). Conclusions. The results of own and literature data indicate that the introduction of testing as a screening for the most common genomic mutations at the preimplantation level is impractical due to high level of mosaicism.

Keywords: preimplantation genetic screening, FISH, aneuploidy, mosaicism, age factor.