Self-incompatibility allele identification in Ukrainian sweet cherry (Prunus avium L.) cultivars

  • Ya. I. Ivanovych Institute of Horticulture NAAS, Ukraine, 03027, Kyiv-27, Novosilky, Sadova str., 23
  • N. V. Tryapitsyna Institute of Horticulture NAAS, Ukraine, 03027, Kyiv-27, Novosilky, Sadova str., 23
  • K. M. Udovychenko Institute of Horticulture NAAS, Ukraine, 03027, Kyiv-27, Novosilky, Sadova str., 23
  • R. A. Volkov Dept. of Molecular Genetics and Biotechnology, Yuri Fedkovych National University of Chernivtsi,Ukraine, 58012, Chernivtsi, Kotsiubynski str., 2


Aim. Ukrainian breeders have created a large number of sweet cherry cultivars, which still remain almost unexplored at the molecular level. The aim of our study was to identify the self-incompatibility alleles (S-alleles) in Ukrainian sweet cherry cultivars and landraces, and to elucidate, to which cross-incompatibility group the cultivars belong. Methods. The PCR was conducted using consensus primers to the first and second introns of S-RNAse gene and to the single intron of SFB gene. The electrophoretic analysis of the PCR products of the second intron of S-RNAse was carried out in agarose gel, whereas detection of fluorescently labeled DNA fragments of the first S-RNAse intron and the SFB intron was performed using a genetic analyzer. Results. The S-alleles of 25 Ukrainian sweet cherry cultivars and 10 landraces were identified. The S-alleles frequencies and affiliation of cultivars and landraces to the groups of cross-incompatibility were determined. The obtained data can be used in breeding programs and by planning of industrial plantings. Conclusions. In the study, 12 different S-alleles and 79 S-haplotypes were identified. The S1, S3, S4, S5, S6 and S9 alleles are the most widespread among Ukrainian sweet cherry cultivars and landraces. The high frequencies of S5 and especially of S9 alleles are characteristic for the Ukrainian cultivars and distinguish them from other European ones. For the Ukrainian sweet cherry cultivars, the XXXVII (S5S9) cross-incompatibility group appeared to be the most numerous.

Keywords: Ukrainian sweet cherry cultivars, S-locus, Sgenotypes, self- and cross-incompatibility, Prunus avium.


Sansavini S., Lugli S. Sweet cherry breeding programs in Europe and Asia. Acta Hortic. 2008. Vol. 795. P. 41–58. doi: 10.17660/ActaHortic.2008.795.1

FAOSTAT database collections. Food and Agriculture Organization of the United Nations. Rome. URL:

Komarova N. Y., Grimm G. W., Hemleben V., Volkov R. A. Molecular evolution of 35S rDNA and taxonomic status of Lycopersicon within Solanum sect. Petota. Plant Syst. Evol. 2008. Vol. 276(1–2). P. 59–71. doi: 10.1007/s00606-008-0091-2

Laidò G., Mangini G., Taranto F., Gadaleta A., Blanco A., Cattivelli L., De Vita P. Genetic diversity and population structure of tetraploid wheats (Triticum turgidum L.) estimated by SSR, DArT and pedigree data. Plos One. 2013. Vol. 8(6). P. 1–17. doi: 10.1371/journal.pone.0067280

Boucheffa S., Miazzi M. M., di Rienzo V., Mangini G., Fanelli V., Tamendjari A., Montemurro C. The coexistence of oleaster and traditional varieties affects genetic diversity and population structure in Algerian olive (Olea europaea) germplasm. Genet. Resour. Crop Evol. 2017. Vol. 64(2). P. 379–390. doi: 10.1007/s10722-016-0365-4

Guarino C., Santoro S., De Simone L., Cipriani G. Prunus avium: nuclear DNA study in wild populations and sweet cherry cultivars. Genome. 2009. Vol. 52(4). P. 320–337. doi: 10.1139/g09-007

Ivanovych Ya. I., Udovychenko K. M., Bublyk M. O., Volkov R. A. ISSR-PCR fingerprinting of Ukrainian sweet cherry (Prunus avium L.) cultivars. Cytology and Genetics. 2017. Vol. 51(1). P. 40–47. doi: 10.3103/S0095452717010066

Ivanovych Ya., Volkov R. Genetic relatedness of sweet cherry (Prunus avium L.) cultivars from Ukraine determined by microsatellite markers. J. Hortic. Sci. Biotechnol. 2017. P. 1–9. doi: 10.1080/14620316.2017.1342568

Cai L., Voorrips R. E., van de Weg E., Peace C., Iezzoni A. Genetic structure of a QTL hotspot on chromosome 2 in sweet cherry indicates positive selection for favorable haplotypes. Mol. Breed. 2017. Vol. 37. P. 1–10. doi: 10.1007/s11032-017-0689-6

Biscarini F., Nazzicari N., Bink M., Arús P., Aranzana M. J., Verde I., Rossini, L. Genome enabled predictions for fruit weight and quality from repeated records in European peach progenies. BMC Genomics. 2017. Vol. 18(1). P. 1–15. doi: 10.1186/s12864-017-3781-8

Ivanovych Ya., Volkov R. Allelic status of PavCNR12 gene in Ukrainian sweet cherry (Prunus avium L.) cultivars. Bul. Vavilov Soc. Geneticists and Breeders of Ukraine. Vol. 15(1). P. 40–46.

Szikriszt B., Doğan A., Ercisli S., Akcay M. E., Hegedűs A., Halász J. Molecular typing of the self-incompatibility locus of Turkish sweet cherry genotypes reflects phylogenetic relationships among cherries and other Prunus species. Tree Genet. Genomes. 2012. Vol. 9(1). P. 155–165. doi: 10.1007/s11295-012-0543-2

Schuster M. Incompatible (S-) genotypes of sweet cherry cultivars (Prunus avium L.). Sci. Hortic. 2012. Vol. 148. P. 59–73. doi: 10.1016/j.scienta.2012.09.012

Cachi A. M., Wünsch A. Characterization and mapping of non-S gametophytic self-compatibility in sweet cherry (Prunus avium L.). J. Exp. Bot. 2011. Vol. 62(6). P. 1847–1856. doi: 10.1093/jxb/erq374

Ikeda K., Watari A., Ushijima K. Molecular markers for the self-compatible S4´-haplotype, a pollen-part mutant in sweet cherry (Prunus avium L.). J. Amer. Soc. Hort. Sci. 2004. Vol. 129(5). P. 724–728.

De Cuyper B., Sonneveld T., Tobutt K. R. Determining self-incompatibility genotypes in Belgian wild cherries. Mol. Ecol. 2005. Vol. 14(4). P. 945–955. doi: 10.1111/j.1365-294X.2005.02460.x

Cabrera A., Rosyara U. R., De Franceschi P., Sebolt A., Sooriyapathirana S. S., Dirlewanger E., van der Knaap E. Rosaceae conserved orthologous sequences marker polymorphism in sweet cherry germplasm and construction of a SNP-based map. Tree Genet. Genomes. 2012. Vol. 8(2). P. 237–247. doi: 10.1007/s11295-011-0436-9

Cachi A. M., Wunsch A., Vilanova A., Guardia M., Ciordia M., Aleta N. S-locus diversity and crosscompatibility of wild Prunus avium for timber breeding. Plant Breed. 2017. Vol. 136(1). P. 126–131. doi: 10.1111/pbr.12450

Marchese A., Giovannini D., Leone A., Mafrica R., Palasciano M., Cantini C., Marra F. P. S-genotype identification, genetic diversity and structure analysis of Italian sweet cherry germplasm. Tree Genet. Genomes. 2017. Vol. 13(5). P. 93. doi: 10.1007/s11295-017-1176-2

Sonneveld T., Tobutt K. R., Robbins T. P. Allelespecific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers. Theor. Appl. Genet. 2003. Vol. 107(6). P. 1059–1070. doi: 10.1007/s00122-003-1274-4

Hegedűs A., Taller D., Papp N., Szikriszt B., Ercisli S., Halász J., Stefanovits-Bányai É. Fruit antioxidant capacity and self-incompatibility genotype of Ukrainian sweet cherry (Prunus avium L.) cultivars highlight their breeding prospects. Euphytica. 2013. Vol. 191(1). P. 153–164. doi: 10.1007/s10681-013-0919-x

Sharma K., Cachi A. M., Sedlák P., Skřivanová A., Wünsch A. S-genotyping of 25 sweet cherry (Prunus avium L.) cultivars from the Czech Republic. J. Hortic. Sci. Biotechnol. 2016. Vol. 91(2). P. 117–121. doi: 10.1080/14620316.2015.1110997

Lisek A., Rozpara E., Głowacka A., Kucharska D., Zawadzka M. Identification of S-genotypes of sweet cherry cultivars from central and Eastern Europe. Hort. Sci. 2015. Vol. 42(1). P. 13–21. doi: 10.17221/103/2014-HORTSCI

Lytovchenko O. M., Pavliuk V. V., Omelchenko I. K. The best varieties of fruit, berry and nut crops of Ukrainian selection. Kyiv : Presa Ukrainy, 2011. 144 p.

Doyle J. J., Doyle J. L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987. Vol. 19. P. 11–15.

Sonneveld T., Robbins T. P., Tobutt K. R. Improved discrimination of self-incompatibility S-RNase alleles in cherry and high throughput genotyping by automated sizing of first intron polymerase chain reaction products. Plant Breed. 2006. Vol. 125(3). P. 305–307. doi: 10.1111/j.1439-0523.2006.01205.x

Vaughan S. P., Russell K., Sargent D. J., Tobutt K. R. Isolation of S-locus F-box alleles in Prunus avium and their application in a novel method to determine selfincompatibility genotype. Theor. Appl. Genet. 2006. Vol. 112(5). P. 856–866. doi: 10.1007/s00122-005-0187-9

Serres-Giardi L., Dufour J., Rusell K., Buret C., Laurens F., Santi F. Natural triploids of wild cherry. Can. J. For. Res. 2010. Vol. 40. P. 1951–1961. doi: 10.1139/X10-100

Ipek A., Gulen H., Akcay M. E., Ipek M., Ergin S., Eris A. Determination of self-incompatibility groups of sweet cherry genotypes from Turkey. Genet. Mol. Res. 2011. Vol. 10(1). P. 253–260. doi: 10.4238/vol10-1gmr1024

Schueler S., Tusch A., Scholz F. Comparative analysis of the within-population genetic structure in wild cherry (Prunus avium L.) at the selfincompatibility locus and nuclear microsatellites. Mol. Ecol. 2006. Vol. 15(11). P. 3231–3243. doi: 10.1111/j.1365-294X.2006.03029.x

Cachi A. M., Wunsch A. S-genotyping of sweet cherry varieties from Spain and S-locus diversity in Europe. Euphytica. 2014. Vol. 197(2). P. 229-236. doi: 10.1007/s10681-014-1061-0