Total reducing capacity in Arabidopsis thaliana cat2cat3 knockout mutants under heat stress

  • I. I. Panchuk Dept. of Molecular Genetics and Biotechnology, Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Kotsubynski str. 2, Chernivtsi
  • I. M. Buzduga Dept. of Molecular Genetics and Biotechnology, Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Kotsubynski str. 2, Chernivtsi
  • R. A. Volkov Dept. of Molecular Genetics and Biotechnology, Yuri Fedkovych National University of Chernivtsi, Ukraine, 58012, Kotsubynski str. 2, Chernivtsi
DOI: https://doi.org/10.7124/visnyk.utgis.15.1.710

Abstract

Aim. It was investigated whether the simultaneous loss of the two catalase isoforms CAT2 and CAT3 can be compensated by the increase in content of low-molecular weight antioxidants. To clarify this question, the total reducing capacity in Arabidopsis wild type and cat2cat3 knockout mutants was evaluated under optimal growth conditions and after heat stress. Methods. Leaves of Arabidopsis thaliana wild type and cat2cat3 knockout mutants were exposed to high temperatures. The content of water-soluble low molecular weight antioxidants was evaluated by determining the total reducing capacity using iodometry. Results. In intact cat2cat3 mutants there is an 1.7 times increase in the content of low-molecular weight antioxidants compared to wild type plants. A high content of these compounds in knockout plants was also observed upon heat stress. Patterns of changes in total reducing capacity differ between wild type and knockout lines. Conclusion. The loss of activity of the catalase isoforms САТ2 and САТ3 in knock-out mutants of Arabidopsis results in activation of non-enzymatic antioxidant defenses. The increase of the content of low-molecular weight antioxidants is one of the mechanisms that provide protection of mutant plants from chronic oxidative stress, both under optimal cultivation conditions and under the influence of elevated temperatures.

Keywords: multigenic family, heat shock, total reducing capacity, knockout mutants, Arabidopsis thaliana.

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