REP-PCR analisis of single agent of cucumber bacterial diseases
Abstract
Aim. For the purpose of correct species identification and estimation of population’s heterogeneity, the fingerprinting of the genome of isolated by us Pectobacterium sp., collection «Erwinia toxica» strains and typical representatives of certain species of Pectobacterium and Diskeya genera has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR), mathematical-statistical methods of research were used. Results. On the basic of BOX, REP and ERIC profiles the significant affinity between isolated Pectobacterium sp. and collections «Erwinia toxica» strains with the typical P. carotovorum susp. carotovorum UCM B1075T has been established. Genetic heterogeneity of isolated Pectobacterium sp. and collections «Erwinia toxica» strains has been estimated. Conclusions. It has been found the significant relationship between isolates Pectobacterium sp. and the collection «Erwinia toxica» strains with the typical strain P. carotovorum susp carotovorum UCM B1075T on the basic of their BOX, REP and ERIC profiles. Most likely, this indicates that they belong to this species. The genetic homogeneity of isolated Pectobacterium sp. strains of and the genetic heterogeneity of the collection «Erwinia toxica» strains is probably due to the plant’s selection from similar or different region.
Keywords: identification, genetic heterogeneity, REPPCR, «Erwinia toxica», Pectobacterium sp.
References
Akhatov A.K., Hannibal F. B., Meshkov Yu. I. et al. Diseases and pests of vegetable crops and potatoes. Moscow: Society of scientific publications KMK, 2013.
Bublik L.I., Vasechko G.I., Vasiliev V.P. et al. Plant protection guide; Ed. M. Plisovy. Kyiv:Urozhai, 1999.
Gvozdyak R.I., Pasichnik L.A., Yakovleva L.M., Moroz S.M., Litvinchuk O.O., Zhytkevich N.V., Hodos S.F., Butsenko L.M., Dankevich L.A., Grinick I.V., Patyka V.P. Phytopathogenic bacteria. Bacterial diseases of plants. Kiev: TOV NVP Interservis, 2011.
Korobko A.P. Biology of pathogens of bacterial cucumbers in the USSR. Author’s abstract. Dis. Kand. biol. science: 03.00.07 / Zabolotny Int. Microbiology and virology. Kiev: 1973. 20 p.
Bergey’s Manual of Systematic Bacteriology. Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York; USA: Springer Science+ Business Media. 2005. Vol. 1. 2nd ed. 1108 p. doi: 10.1007/0-387-28022-7
Ozturk M., Aksoy H.M., Ozturk S., Potrykus M., Lojkowska E. First report of potato blackleg and soft rot caused by Pectobacterium wasabiae in Turkey. New Disease Reports. 2016. Vol. 34. P. 17. doi: 10.5197/j.2044-0588.2016.034.017
Rosenzweig N., Steere L., Kirk W. W., Mambetova S., Long C., Schafer R., Dangi S., Byrne J. First report of Dickeya dianthicola and Pectobacterium wasabiae causing aerial stem rot of potato in Michigan, USA. New Disease Reports. 2016. Vol. 33. P. 10. doi: 10.5197/j.2044-0588.2016.033.010
Mikicinski A., Sobiczewski P., Sulikowska M., Pulawska J., Treder J. Pectolytic bacteria associated with soft rot of calla lily (Zantedeschia spp.) tubers. J Phytopathol. 2010. Vol. 158. P. 201–209. doi: 10.1111/j.1439-0434.2009.01597.x
Czajkowski R., Pérocmbelon M. C. M, Jafra S., Lojkowska E., Potrykus M., J. M., van der Wolf, Sledz W. Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review. Annals of Applied Biology. 2015. Vol. 166(1). P. 1–21. doi: 10.1111/aab.12166
Aremu B. R., Babalola O. O. Classification and taxonomy of vegetable macergens. Front. Microbiol. 2016. Vol. 6. P. 1361–1372. doi: 10.3389/fmicb.2015.01361
Tavasoli E., Marefat A. R., Hassanzadeh N. Identity and genetic diversity of Pectobacterium spp., causal agents of potato soft rot in Zanjan, Iran. African Journal of Plant Science. 2011. Vol. 5(6). P. 329–336.
Louws F. J., Rademaker J. L. W, de Bruijn F. J. The three DS of PCR−based genomic analysis of phytobacteria: Diversity, detection, and disease diagnosis. Annual Reviews Phytopathology. 1999. Vol. 37. P. 81−125. doi: 10.1146/annurev.phyto.37.1.81
Faquihi H., Terta M., Amdan M., Achbani El H., Ennaji M. M., Mhand R. A. Phenotypic and genotypic diversity of Pectobacterium carotovorum subsp carotovorum causing soft rot disease of potatoes in Morocco. Eur J Plant Pathol. 2015. Vol. 143(4). P. 801−811. doi: 10.1007/s10658-015-0732-6
Louws F. J., Fulbright D. W., Stephens C. T. Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Applied and Enviromental Microbiology. 1994. Vol. 60(7). P. 2286−2295.
Rademaker J. LW, de Bruijn F. J. Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. DNA markers: protocols, applications and overviews. 1997. Vol. 1. P. 151−171.
Buonaurio R., Caglioti C., Marques Pires M., Moretti Ch., Innocenti M. Occurrence of a soft rot of calla (Zantedeschia aethiopica) caused by Pectobacterium carotovorum subsp. carotovorum in central Italy. Phytopathol. Mediterr. 2002. Vol. 41(2). P. 152–156. doi: 10.14601/Phytopathol_Mediterr-1667
Kazemi F., Khodakaramian Gh., Bagheri A and Ghasemi A. Rep−PCR pattern of the strains of Pectobacterium isolated from potato soft rot and black leg diseases in Hamedan province. Agricultural biotechnology. 2011. Vol. 10(1). P. 47−53.
Dadasoglu F., Kotan R. Identification and characterization of Pectobacterium carotovorum. The Journal of Animal and Plant Sciences. 2017. Vol. 27(2). P. 647−654.
Rafiei S., Khodakaramian Gh., Baghaee-Ravari S. Characterization of Pectobacterium species isolated from vegetable crops in north-west of Iran. African Journal of Agricultural Research., 2015. Vol. 10(46). P. 4258−4267.
