Genetic diversity in Gentiana lutea L. populations from Svydivets ridge of the Ukrainian Carpathians
Aim. To evaluate the levels of intra- and interpopulation diversity in G. lutea from Svydivets ridge of the Ukrainian Carpathians using PCR-markers. Methods. Genetic variability of plants from two populations was assessed by RAPD-, ISSR-, CDDP- and RGAP- markers. Profiles of PCR products were recorded as binary matrices, which were further analyzed to determine the proportion of polymorphic amplicons (P), expected heterozygosity (He), Shannon index (S), and genetic distances by Jaccard (Dj). The distribution of total genetic variation between and within the studied populations was quantified using the analysis of molecular variance (AMOVA). Results. The range of indices of genetic diversity in both populations calculated for different types of markers was as follows: P – 35.0–56.7%, He – 0.113–0.176, S – 0.170–0.267, Dj – 16.1–37.3%. The samples on the dendrogram of genetic similarity and based on the results of analysis of principal coordinates (PCoA) were grouped according to their population of origin. AMOVA results showed that the differences between populations by RAPD-, RGAP-, CDDP-analyzes accounted for approximately 70%; and by ISSR-analysis made up roughly 60% of the total genetic variation, while intrapopulation polymorphism was around 30 and 40%, respectively. Conclusions. The indices of genetic diversity were the same in two studied populations of G. lutea from Svydivets ridge, and were similar with the indices of populations from the Chornogora ridge of the Ukrainian Carpathians. The large variation between the studied populations revealed by the AMOVA indicates significant isolation and differentiation of the populations.
Key words: Gentiana lutea L., genetic diversity, PCR markers, intra- and interpopulation variation, analysis of molecular variance (AMOVA).