Relationship between cell wall fatty acid composition of the yeast strain Kluyveromyces marxianus UKM Y-305 and 2-phenylethanol and ethanol production

  • О. Г. Мамєєва Zabolotny Institute of Microbiology and Virology, NAS of Ukraine Ukraine, D03680, Kyiv, Akademika Zabolotnoho str., 154


Aim. Aim of this work was to study the fatty acid (FA) composition of the cell wall in the yeast Kluyveromyces marxianus UKM Y-305 under different cultivation parameters and determine the relationship between its FA composition and producing of 2-phenylethanol and ethanol. Methods. Yeast growth and cultivation parameters were studied using conventional microbiological methods. Concentrations of 2-phenylethanol, ethanol and crystal structure of cellular lipids were explored by chromatography-mass spectrometry. Results. The fatty acid composition of the cell wall in K. marxianus UCM Y-305 yeast was determined. High concentrations of oleic acid in the cell wall of K. UKM marxianus Y-305 yeast was shown to ensure their adaptation to the toxic effects of ethanol and 2-phenylethanol. Conclusions. K. marxianus UCM Y-305 yeast strain is significantly resistant to ethanol and 2-phenylethanol stress.

Key words : Kluyveromyces, fatty acid, 2-phenylethanol, ethanol, toxicity.