Relationship between cell wall fatty acid composition of the yeast strain Kluyveromyces marxianus UKM Y-305 and 2-phenylethanol and ethanol production
Aim. Aim of this work was to study the fatty acid (FA) composition of the cell wall in the yeast Kluyveromyces marxianus UKM Y-305 under different cultivation parameters and determine the relationship between its FA composition and producing of 2-phenylethanol and ethanol. Methods. Yeast growth and cultivation parameters were studied using conventional microbiological methods. Concentrations of 2-phenylethanol, ethanol and crystal structure of cellular lipids were explored by chromatography-mass spectrometry. Results. The fatty acid composition of the cell wall in K. marxianus UCM Y-305 yeast was determined. High concentrations of oleic acid in the cell wall of K. UKM marxianus Y-305 yeast was shown to ensure their adaptation to the toxic effects of ethanol and 2-phenylethanol. Conclusions. K. marxianus UCM Y-305 yeast strain is significantly resistant to ethanol and 2-phenylethanol stress.
Key words : Kluyveromyces, fatty acid, 2-phenylethanol, ethanol, toxicity.